Evidence map›Paper›PMID 36985761›Full record

ArticleMolecules (Basel, Switzerland)2023

Comparison of Synthetic Methods and Identification of Several Artificial Antigens of Deoxynivalenol.

Li Han, Yuetao Li, Jinqing Jiang, Changzhong Liu, Jie Hou, Bo Wang, Ziliang Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Li HanHenan Institute of Science and Technology, College of Animal Science and Veterinary Medicine, Xinxiang 453003, China.
Yuetao LiHenan Institute of Science and Technology, College of Animal Science and Veterinary Medicine, Xinxiang 453003, China.
Jinqing JiangHenan Institute of Science and Technology, College of Animal Science and Veterinary Medicine, Xinxiang 453003, China.ORCID 0000-0003-0683-0331
Changzhong LiuHenan Institute of Science and Technology, College of Animal Science and Veterinary Medicine, Xinxiang 453003, China.
Jie HouHenan Institute of Science and Technology, College of Animal Science and Veterinary Medicine, Xinxiang 453003, China.
Bo WangHenan Institute of Science and Technology, College of Animal Science and Veterinary Medicine, Xinxiang 453003, China.
Ziliang WangHenan Institute of Science and Technology, College of Animal Science and Veterinary Medicine, Xinxiang 453003, China.

Funding

the 2022 innovation and entrepreneurship training program for College Students of Henan Institute of Science and Technology 2022CX035the 2023 Key Scientific Research Project of Universities in Henan Province 23B230001the introduction of leading talents and the construction of high-level talents team of Henan Institute of Science and Technology (doctoral research initiation fee) 103020221002/010the Program for Innovative Research Team (in Science and Technology) in University of Henan Province 20IRTSTHN025the Twelfth Five-Year Plan of National Science and Technology Support Projects, "Research and Demonstration of Rapid Detection Technology for Hormone Drugs" 2014BAD13B05-01
6 · The paper itself

Abstract

The purpose of this experiment was to study the design and modification of hapten molecules and artificial antigen molecules of deoxynivalenol (DON), and to compare the preparation and identification methods of four artificial antigens. According to the characteristics of the molecular structure of DON, four artificial antigen coupling methods were designed-namely, N,N'-carbonyldiimidazole (CDI), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), isobutyl chloroformate (IBCF), and N-hydroxysuccinimide (NHS)-to prepare artificial antigens and detection antigens. Through ultraviolet (UV), infrared (IR), and SDS-polyacrylamide gel electrophoresis (SDS-PAGE), along with other physical and chemical identification methods and animal immunisation, the best artificial antigen coupling method was screened. The results showed that the CDI method achieved the best effect among the synthesis methods. The titre of anti-DON polyclonal antibody (pAb) produced by animal immunisation reached 1: (6.4 × 10

Indexed as

AntigensHaptensAnimalsMolecular StructureTrichothecenesVaccines, SyntheticAntigensdeoxynivalenolHaptensTrichothecenesVaccines, Syntheticartificial antigendeoxynivalenolN,N′-carbonyldiimidazole

Identifiers

PMID36985761
PMCPMC10055668

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.