Evidence map›Paper›PMID 36985424›Full record

ReviewMolecules (Basel, Switzerland)2023

Photoactivatable BODIPYs for Live-Cell PALM.

Yang Zhang, Yeting Zheng, Andrea Tomassini, Ambarish Kumar Singh, Françisco M Raymo

Abstract readReview
In one paragraph

Review in Molecules (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Photoactivation of BODIPY Fluorescence with Green Light.The Journal of organic chemistry · 2025
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yang ZhangProgram of Polymer and Color Chemistry, Department of Textile Engineering, Chemistry and Science, North Carolina State University, Raleigh, NC 27606, USA.
Yeting ZhengLaboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146-0431, USA.
Andrea TomassiniLaboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146-0431, USA.ORCID 0000-0002-1386-4866
Ambarish Kumar SinghLaboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146-0431, USA.
Françisco M RaymoLaboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146-0431, USA.ORCID 0000-0002-6163-6606

Funding

Photoactivatable Fluorophores for High-Throughput Multiplexed Tracking of Single-Molecules in Live CellsR01GM143397 · NIGMS · UNIVERSITY OF MIAMI CORAL GABLES · PI RAYMO, FRANCISCO M · 2022 to 2025
$1.4M
NIGMS NIH HHS R01 GM143397NIH HHS NIGMS-R01GM143397NIH HHS NIGMS-R21GM141675
6 · The paper itself

Abstract

Photoactivated localization microscopy (PALM) relies on fluorescence photoactivation and single-molecule localization to overcome optical diffraction and reconstruct images of biological samples with spatial resolution at the nanoscale. The implementation of this subdiffraction imaging method, however, requires fluorescent probes with photochemical and photophysical properties specifically engineered to enable the localization of single photoactivated molecules with nanometer precision. The synthetic versatility and outstanding photophysical properties of the borondipyrromethene (BODIPY) chromophore are ideally suited to satisfy these stringent requirements. Specifically, synthetic manipulations of the BODIPY scaffold can be invoked to install photolabile functional groups and photoactivate fluorescence under photochemical control. Additionally, targeting ligands can be incorporated in the resulting photoactivatable fluorophores (PAFs) to label selected subcellular components in live cells. Indeed, photoactivatable BODIPYs have already allowed the sub-diffraction imaging of diverse cellular substructures in live cells using PALM and can evolve into invaluable analytical probes for bioimaging applications.

Indexed as

Boron CompoundsFluorescent DyesMicroscopy, Fluorescence4,4-difluoro-4-bora-3a,4a-diaza-s-indaceneBoron CompoundsFluorescent Dyesborondipyrromethenes (BODIPYs)fluorescence imagingphotoactivatable fluorophores (PAFs)photoactivated localization microscopy (PALM)single-molecule localization microscopy (SMLM)

Identifiers

PMID36985424
PMCPMC10057988

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.