Evidence map›Paper›PMID 36985386›Full record

ArticleMicroorganisms2023

Rapid Reverse Purification DNA Extraction Approaches to Identify Microbial Pathogens in Wastewater.

Sarah Schurig, Rea Kobialka, Andy Wende, Md Anik Ashfaq Khan, Phillip Lübcke, Elias Eger, Katharina Schaufler, Arwid Daugschies, Uwe Truyen, Ahmed Abd El Wahed

Open access · goldAbstract read
In one paragraph

Article in Microorganisms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Recent Developments onFoods (Basel, Switzerland) · 2025
    Review
  3. Article
  4. Detection ofMicroorganisms · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Sarah SchurigInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04103 Leipzig, Germany.
Rea KobialkaInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04103 Leipzig, Germany.ORCID 0009-0007-5114-1910
Andy WendeXpedite Diagnostics GmbH, 80687 Munich, Germany.
Md Anik Ashfaq KhanInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04103 Leipzig, Germany.
Phillip LübckeInstitute of Pharmacy, University of Greifswald, 17489 Greifswald, Germany.
Elias EgerInstitute of Infection Medicine, Christian-Albrecht University Kiel, 24105 Kiel, Germany.ORCID 0000-0002-5514-8083
Katharina SchauflerInstitute of Pharmacy, University of Greifswald, 17489 Greifswald, Germany.ORCID 0000-0002-2669-8799
Arwid DaugschiesInstitute of Parasitology, Centre for Infectious Disease, Leipzig University, 04103 Leipzig, Germany.
Uwe TruyenInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04103 Leipzig, Germany.
Ahmed Abd El WahedInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04103 Leipzig, Germany.ORCID 0000-0003-3347-6075
Leipzig University · DEChristian-Albrechts-Universität zu Kiel · DECS Diagnostics · DEUniversität Greifswald · DE

Funding

Federal Ministry of Education and Research NA
6 · The paper itself

Abstract

Wastewater monitoring became a promising solution in the early detection of outbreaks. Despite the achievements in the identification of pathogens in wastewater using real-time PCR, there is still a lack of reliable rapid nucleic acid extraction protocols. Therefore, in this study, samples were subjected to alkali, proteinase K and/or bead-beating followed by reverse purification magnetic beads-based separation. Wastewater samples spiked with

Indexed as

DNA extractionreverse purificationsurveillancewastewater

Identifiers

PMID36985386
PMCPMC10056086
OpenAlexW4353065772

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.