Evidence map›Paper›PMID 36985113›Full record

ArticleMicromachines2023

Dielectrophoresis-Based Selective Droplet Extraction Microfluidic Device for Single-Cell Analysis.

Seito Shijo, Daiki Tanaka, Tetsushi Sekiguchi, Jun-Ichi Ishihara, Hiroki Takahashi, Masashi Kobayashi, Shuichi Shoji

Abstract read
In one paragraph

Article in Micromachines, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Seito ShijoMajor in Nanoscience and Nanoengineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo 145-0065, Japan.
Daiki TanakaResearch Organization for Nano & Life Innovation, Waseda University, 513 Tsurumakicho, Shinjuku, Tokyo 162-0041, Japan.ORCID 0000-0003-1900-4848
Tetsushi SekiguchiResearch Organization for Nano & Life Innovation, Waseda University, 513 Tsurumakicho, Shinjuku, Tokyo 162-0041, Japan.
Jun-Ichi IshiharaMedical Mycology Research Center, Chiba University, 181 Inohana, Chuo, Chiba 260-8673, Japan.
Hiroki TakahashiMedical Mycology Research Center, Chiba University, 181 Inohana, Chuo, Chiba 260-8673, Japan.ORCID 0000-0001-5627-1035
Masashi KobayashiMajor in Nanoscience and Nanoengineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo 145-0065, Japan.
Shuichi ShojiMajor in Nanoscience and Nanoengineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo 145-0065, Japan.

Funding

Japan Ministry of Education, Culture, Sports Science & Technology (MEXT), Grant-in-Aid for Early-Career Scientists No. 22K15152Japan Ministry of Education, Culture, Sports Science & Technology (MEXT), Grant-in-Aid for Scientific Basic Research No. 20H00336
6 · The paper itself

Abstract

We developed a microfluidic device that enables selective droplet extraction from multiple droplet-trapping pockets based on dielectrophoresis. The device consists of a main microchannel, five droplet-trapping pockets with side channels, and drive electrode pairs appropriately located around the trapping pockets. Agarose droplets capable of encapsulating biological samples were successfully trapped in the trapping pockets due to the difference in flow resistance between the main and side channels. Target droplets were selectively extracted from the pockets by the dielectrophoretic force generated between the electrodes under an applied voltage of 500 V. During their extraction from the trapping pockets, the droplets and their contents were exposed to an electric field for 400-800 ms. To evaluate whether the applied voltage could potentially damage the biological samples, the growth rates of

Indexed as

dielectrophoresis (DEP)microdropletmicrofluidicsselective extraction

Identifiers

PMID36985113
PMCPMC10058699

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.