Evidence map›Paper›PMID 36982762›Full record

ArticleInternational journal of molecular sciences2023

Botulinum Neurotoxin A4 Has a 1000-Fold Reduced Potency Due to Three Single Amino Acid Alterations in the Protein Receptor Binding Domain.

William H Tepp, Marite Bradshaw, Alexander P Gardner, Rebecca L Kaufman, Joseph T Barbieri, Sabine Pellett

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

William H TeppDepartment of Bacteriology, University of Wisconsin, Madison, WI 53706, USA.
Marite BradshawDepartment of Bacteriology, University of Wisconsin, Madison, WI 53706, USA.
Alexander P GardnerDepartment of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA.ORCID 0000-0003-4503-1145
Rebecca L KaufmanDepartment of Bacteriology, University of Wisconsin, Madison, WI 53706, USA.
Joseph T BarbieriDepartment of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Sabine PellettDepartment of Bacteriology, University of Wisconsin, Madison, WI 53706, USA.ORCID 0000-0001-8186-8810
University of Wisconsin–Madison · USMedical College of Wisconsin · US

Funding

Characteristics of Botulinum Neurotoxins the Determine PotencyR01AI139306 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI Joseph T Barbieri, Sabine Pellett · 2019 to 2026
$5.5M
NIAID NIH HHS R01 AI139306
6 · The paper itself

Abstract

Botulinum neurotoxin subtype A4 (BoNT/A4) is ~1000-fold less potent than BoNT/A1. This study addresses the basis for low BoNT/A4 potency. Utilizing BoNT/A1-A4 and BoNT/A4-A1 Light Chain-Heavy Chain (LC-HC) chimeras, HC-A4 was responsible for low BoNT/A4 potency. Earlier studies showed BoNT/A1-receptor binding domain (Hcc) bound a β-strand peptide (556-564) and glycan-N

Indexed as

Amino AcidsHumansProtein BindingProtein DomainsAmino AcidsBoNTBoNT/A4botulinum neurotoxinclostridium botulinumpotencyreceptorsubtypesSV2C

Identifiers

PMID36982762
PMCPMC10055998
OpenAlexW4327731217

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.