Evidence map›Paper›PMID 36980946›Full record

ArticleGenes2023

Selection and Validation of Reference Genes for Gene Expression Studies in an Equine Adipose-Derived Mesenchymal Stem Cell Differentiation Model by Proteome Analysis and Reverse-Transcriptase Quantitative Real-Time PCR.

Angela L Riveroll, Sabrina Skyba-Lewin, K Devon Lynn, Glady's Mubyeyi, Ahmad Abd-El-Aziz, Frederick S T Kibenge, Molly J T Kibenge, Alejandro M Cohen, Blanca Esparza-Gonsalez, Laurie McDuffee and 1 more

Open access · goldFull text read
In one paragraph

Article in Genes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Angela L RiverollDepartment of Applied Human Sciences, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada.ORCID 0000-0002-2338-6626
Sabrina Skyba-LewinDepartment of Biology, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
K Devon LynnDepartment of Biology, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
Glady's MubyeyiDepartment of Biology, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
Ahmad Abd-El-AzizDepartment of Biology, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.ORCID 0000-0002-6741-0755
Frederick S T KibengeDepartment of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.ORCID 0000-0002-3852-282X
Molly J T KibengeDepartment of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.ORCID 0000-0003-3406-3766
Alejandro M CohenBiological Mass Spectrometry Core Facility, Dalhousie University, Halifax, NS B3H 4R2, Canada.ORCID 0000-0001-8646-8525
Blanca Esparza-GonsalezDepartment of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
Laurie McDuffeeDepartment of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.ORCID 0000-0002-5095-5329
William J MontelpareDepartment of Applied Human Sciences, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada.ORCID 0000-0002-4167-4613
University of Prince Edward Island · CADalhousie University · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adipose-derived stem cells (ADSCs) are used in tissue regeneration therapies. The objective of this study is to identify stable reference genes (RGs) for use in gene expression studies in a characterized equine adipose-derived mesenchymal stem cell (EADMSC) differentiation model. ADSCs were differentiated into adipocytes (ADs) or osteoblasts (OBs), and the proteomes from these cells were analyzed by liquid chromatography tandem mass spectrometry. Proteins that were stably expressed in all three cells types were identified, and the mRNA expression stabilities for their corresponding genes were validated by RT-qPCR.

Indexed as

Mesenchymal Stem CellsProteomeAnimalsCell DifferentiationDNA-Directed RNA PolymerasesGene ExpressionHorsesReal-Time Polymerase Chain ReactionRNA-Directed DNA PolymeraseRNA, MessengerDNA-Directed RNA PolymerasesProteomeRNA-Directed DNA PolymeraseRNA, MessengerACTBCCDC97EPHA2equine adipose-derived mesenchymal stem cell differentiationFABP5gene expressionPPP6R1protein expressionreference genesRUNX2

Identifiers

PMID36980946
PMCPMC10048155
OpenAlexW4323668929

What OpenQuestion holds

Textfull text, public
LicenceCC BY
reference markers read5
measurements read90
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.