Evidence map›Paper›PMID 36979966›Full record

ArticleBiomedicines2023

Adipose-Derived Stromal Cells within a Gelatin Matrix Acquire Enhanced Regenerative and Angiogenic Properties: A Pre-Clinical Study for Application to Chronic Wounds.

Nicolo Costantino Brembilla, Ali Modarressi, Dominik André-Lévigne, Estelle Brioudes, Florian Lanza, Hubert Vuagnat, Stéphane Durual, Laurine Marger, Wolf-Henning Boehncke, Karl-Heinz Krause and 1 more

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In one paragraph

Article in Biomedicines, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Nicolo Costantino BrembillaDepartment of Pathology and Immunology, Faculty of Medicine, University of Geneva, 1205 Geneva, Switzerland.
Ali ModarressiDivision of Plastic, Reconstructive and Aesthetic Surgery, Geneva University Hospitals, 1205 Geneva, Switzerland.
Dominik André-LévigneDivision of Plastic, Reconstructive and Aesthetic Surgery, Geneva University Hospitals, 1205 Geneva, Switzerland.ORCID 0000-0002-0396-7511
Estelle BrioudesLaboratory of Therapy and Stem Cells, Geneva University Hospitals, 1205 Geneva, Switzerland.
Florian LanzaDepartment of Pathology and Immunology, Faculty of Medicine, University of Geneva, 1205 Geneva, Switzerland.
Hubert VuagnatProgram for Wounds and Wound Healing, Geneva University Hospitals, 1205 Geneva, Switzerland.
Stéphane DurualLaboratory of Biomaterials, Faculty of Dental Medicine, University of Geneva, 1205 Geneva, Switzerland.ORCID 0000-0002-9772-8406
Laurine MargerLaboratory of Biomaterials, Faculty of Dental Medicine, University of Geneva, 1205 Geneva, Switzerland.
Wolf-Henning BoehnckeDepartment of Pathology and Immunology, Faculty of Medicine, University of Geneva, 1205 Geneva, Switzerland.
Karl-Heinz KrauseDepartment of Pathology and Immunology, Faculty of Medicine, University of Geneva, 1205 Geneva, Switzerland.ORCID 0000-0002-9033-6768
Olivier Preynat-SeauveLaboratory of Therapy and Stem Cells, Geneva University Hospitals, 1205 Geneva, Switzerland.
University of Geneva · CHUniversity Hospital of Geneva · CH

Funding

Carigest SA no grant numberFondation Louis Jeantet-Fondation privée des Hopitaux Universitaires de Genève no grant numberFondation pour la recherche sur le diabète no grant numberInnogap no grant numberInnosuisse 43333.1
6 · The paper itself

Abstract

This study evaluates the influence of a gelatin sponge on adipose-derived stromal cells (ASC). Transcriptomic data revealed that, compared to ASC in a monolayer, a cross-linked porcine gelatin sponge strongly influences the transcriptome of ASC. Wound healing genes were massively regulated, notably with the inflammatory and angiogenic factors. Proteomics on conditioned media showed that gelatin also acted as a concentrator and reservoir of the regenerative ASC secretome. This secretome promoted fibroblast survival and epithelialization, and significantly increased the migration and tubular assembly of endothelial cells within fibronectin. ASC in gelatin on a chick chorioallantoic membrane were more connected to vessels than an empty sponge, confirming an increased angiogenesis in vivo. No tumor formation was observed in immunodeficient nude mice to which an ASC gelatin sponge was transplanted subcutaneously. Finally, ASC in a gelatin sponge prepared from outbred rats accelerated closure and re-vascularization of ischemic wounds in the footpads of rats. In conclusion, we provide here preclinical evidence that a cross-linked porcine gelatin sponge is an optimal carrier to concentrate and increase the regenerative activity of ASC, notably angiogenic. This formulation of ASC represents an optimal, convenient and clinically compliant option for the delivery of ASC on ischemic wounds.

Indexed as

adipose stem cellsclinical translationmesenchymal stem cellsstromal cells

Identifiers

PMID36979966
PMCPMC10046849
OpenAlexW4360613986

What OpenQuestion holds

Textfull text, public
LicenceCC BY
measurements read48
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.