ArticleBiosensors2023
Odorant Binding Causes Cytoskeletal Rearrangement, Leading to Detectable Changes in Endothelial and Epithelial Barrier Function and Micromotion.
Article in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 5 citations in OpenAlex.
- A review of research advances in the modulation of olfactory receptors for COPD inflammation and airway remodeling.Frontiers in immunology · 2025Review
- Olfactory Receptors and Aortic Aneurysm: Review of Disease Pathways.Journal of clinical medicine · 2024Review
- Deciphering olfactory receptor binding mechanisms: a structural and dynamic perspective on olfactory receptors.Frontiers in molecular biosciences · 2024Review
- Predictive Cell Culture Time Evolution Based on Electric Models.Biosensors · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Non-olfactory cells have excellent biosensor potential because they express functional olfactory receptors (ORs) and are non-neuronal cells that are easy to culture. ORs are G-protein coupled receptors (GPCRs), and there is a well-established link between different classes of G-proteins and cytoskeletal structure changes affecting cellular morphology that has been unexplored for odorant sensing. Thus, the present study was conducted to determine if odorant binding in non-olfactory cells causes cytoskeletal changes that will lead to cell changes detectable by electric cell-substrate impedance sensing (ECIS). To this end, we used the human umbilical vein endothelial cells (HUVECs), which express OR10J5, and the human keratinocyte (HaCaT) cells, which express OR2AT4. Using these two different cell barriers, we showed that odorant addition, lyral and Sandalore, respectively, caused an increase in cAMP, changes in the organization of the cytoskeleton, and a decrease in the integrity of the junctions between the cells, causing a decrease in cellular electrical resistance. In addition, the random cellular movement of the monolayers (micromotion) was significantly decreased after odorant exposure. Collectively, these data demonstrate a new physiological role of olfactory receptor signaling in endothelial and epithelial cell barriers and represent a new label-free method to detect odorant binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.