ArticleBiomolecules2023
I Choose You: Selecting Accurate Reference Genes for qPCR Expression Analysis in Reproductive Tissues in
Article in Biomolecules, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- Different Dynamics:Biology · 2026Article
- Selection and validation of suitable reference genes for qPCR expression analysis in youngFrontiers in plant science · 2026Article
- Development of functional markers and expression analysis for a Peroxidase gene TaPod-A3 on chromosome 7AL in wheat.The plant genome · 2025Article
- Discovery and validation of a novel dual-target blood test for the detection of hepatocellular carcinoma across stages from cirrhosis.BMC medicine · 2025Article
- Artificial Sweet Cherry miRNA 396 Promotes Early Flowering in Vernalization-Dependent Arabidopsis Edi-0 Ecotype.Plants (Basel, Switzerland) · 2025Article
- Selection and validation of reference genes for quantitative real-time PCR during the developmental stages of seeds inFrontiers in plant science · 2025Article
- Transcriptomic landscape of seedstick in Arabidopsis thaliana funiculus after fertilisation.BMC plant biology · 2024Article
- Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Reference Genes Screening and Gene Expression Patterns Analysis Involved in Gelsenicine Biosynthesis under Different Hormone Treatments inInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time polymerase chain reaction (qPCR) is a widely used method to analyse the gene expression pattern in the reproductive tissues along with detecting gene levels in mutant backgrounds. This technique requires stable reference genes to normalise the expression level of target genes. Nonetheless, a considerable number of publications continue to present qPCR results normalised to a single reference gene and, to our knowledge, no comparative evaluation of multiple reference genes has been carried out in specific reproductive tissues of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.