Evidence map›Paper›PMID 36978564›Full record

ArticleAnimals : an open access journal from MDPI2023

Effects of Trehalose Supplementation on Lipid Composition of Rooster Spermatozoa Membranes in a Freeze/Thaw Protocol.

Olga I Stanishevskaya, Yulia Silyukova, Elena Fedorova, Nikolai Pleshanov, Anton Kurochkin, Vera M Tereshina, Elena Ianutsevich

Open access · goldFull text read
In one paragraph

Article in Animals : an open access journal from MDPI, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
4.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Cold adaptation mechanisms of the psychrotolerant filamentous ascomycete Psychrophilomyces antarcticus.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Olga I StanishevskayaRussian Research Institute of Farm Animal Genetics and Breeding-Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, 55a, Pushkin, 196625 St. Petersburg, Russia.
Yulia SilyukovaRussian Research Institute of Farm Animal Genetics and Breeding-Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, 55a, Pushkin, 196625 St. Petersburg, Russia.ORCID 0000-0003-1905-6373
Elena FedorovaRussian Research Institute of Farm Animal Genetics and Breeding-Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, 55a, Pushkin, 196625 St. Petersburg, Russia.ORCID 0000-0002-1618-6271
Nikolai PleshanovRussian Research Institute of Farm Animal Genetics and Breeding-Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, 55a, Pushkin, 196625 St. Petersburg, Russia.
Anton KurochkinRussian Research Institute of Farm Animal Genetics and Breeding-Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, 55a, Pushkin, 196625 St. Petersburg, Russia.
Vera M TereshinaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.
Elena IanutsevichWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.
All-Russian Research Institute of Genetics and Farm Animal Breeding · RURussian Academy of Sciences · RU

Funding

Russian Science Foundation 19-16-00009-P
6 · The paper itself

Abstract

The plasma membrane of spermatozoa plays an important role in the formation and maintenance of many functions of spermatozoa, including during cryopreservation. As a result of chromatographic analysis, the content of lipids and fatty acids in the membranes of spermatozoa of roosters of two breeds was determined under the influence of cryoprotective media containing trehalose LCM-control (0 mM), Treh20 (9.5 mM), and Treh30 (13.4 mM). The use of the cryoprotective diluent Treh20 made it possible to maintain a dynamic balance between the synthesis and degradation of phospholipids and sterols in the plasma membranes of frozen/thawed spermatozoa, close to that of native spermatozoa. This contributed to an increase in the preservation of frozen/thawed spermatozoa membranes from 48.3% to 52.2% in the egg breed and from 30.0% to 35.1% in the meat- and-egg breed. It was also noted that their kinetic apparatus (mobility indicators) remained at the level of 45.6% (egg breed) and 52.4% (meat-and-egg breed). An increase in the concentration of trehalose to 13.4 mM in a cryoprotective diluent for rooster sperm resulted in a decrease in the morphofunctional parameters of frozen/thawed spermatozoa.

Indexed as

cryopreservationfatty acidsmembrane lipidsroosterspermatozoatrehalose

Identifiers

PMID36978564
PMCPMC10044598
OpenAlexW4324090987

What OpenQuestion holds

Textfull text, public
LicenceCC BY
measurements read83
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.