Evidence map›Paper›PMID 36978544›Full record

ArticleAnimals : an open access journal from MDPI2023

Advancing DNA Barcoding to Elucidate Elasmobranch Biodiversity in Malaysian Waters.

Kar-Hoe Loh, Kean-Chong Lim, Amy Yee-Hui Then, Serena Adam, Amanda Jhu-Xhin Leung, Wenjia Hu, Chui Wei Bong, Aijun Wang, Ahemad Sade, Jamil Musel and 1 more

Full text read
In one paragraph

Article in Animals : an open access journal from MDPI, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Kar-Hoe LohInstitute of Ocean and Earth Sciences, Universiti Malaya, Kuala Lumpur 50603, Malaysia.ORCID 0000-0001-8406-6485
Kean-Chong LimInstitute of Ocean and Earth Sciences, Universiti Malaya, Kuala Lumpur 50603, Malaysia.
Amy Yee-Hui ThenInstitute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia.ORCID 0000-0001-6558-8237
Serena AdamWorld Wide Fund for Nature Malaysia, Petaling Jaya 46150, Malaysia.
Amanda Jhu-Xhin LeungInstitute of Ocean and Earth Sciences, Universiti Malaya, Kuala Lumpur 50603, Malaysia.ORCID 0000-0002-4416-001X
Wenjia HuKey Laboratory of Marine Ecological Conservation and Restoration, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China.
Chui Wei BongInstitute of Ocean and Earth Sciences, Universiti Malaya, Kuala Lumpur 50603, Malaysia.ORCID 0000-0001-7859-4759
Aijun WangFujian Provincial Key Laboratory of Marine Physical and Geological Processes, Xiamen 361005, China.
Ahemad SadeDepartment of Fisheries Sabah, Kota Kinabalu 88624, Malaysia.
Jamil MuselFisheries Research Institute Sarawak, Department of Fisheries Malaysia, Kuching 93744, Malaysia.
Jianguo DuKey Laboratory of Marine Ecological Conservation and Restoration, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China.ORCID 0000-0002-8722-903X

Funding

China-ASEAN Maritime Cooperation fund project "Marine Protected Areas Network in Chi-na-ASEAN Countries" IF030B-2019Monitoring and Conservation of the coastal ecosystem in the Soutth China Sea; National Natural Science foundation of China No: 41961144022Third Institute of Oceanography, Ministry of Natural Resources, China IF004-2022Universiti Malaya grants BK018-2015, RP018C-16SUSCWWF- Malaysia PV049-2019 and PV023-2022
6 · The paper itself

Abstract

The data provided in this article are partial fragments of the Cytochrome c oxidase subunit 1 mitochondrial gene (CO1) sequences of 175 tissues sampled from sharks and batoids collected from Malaysian waters, from June 2015 to June 2022. The barcoding was done randomly for six specimens from each species, so as to authenticate the code. We generated barcodes for 67 different species in 20 families and 11 orders. DNA was extracted from the tissue samples following the Chelex protocols and amplified by polymerase chain reaction (PCR) using the barcoding universal primers FishF2 and FishR2. A total of 654 base pairs (bp) of barcode CO1 gene from 175 samples were sequenced and analysed. The genetic sequences were blasted into the NCBI GenBank and Barcode of Life Data System (BOLD). A review of the blast search confirmed that there were 68 valid species of sharks and batoids that occurred in Malaysian waters. We provided the data of the COI gene mid-point rooting phylogenetic relation trees and analysed the genetic distances among infra-class and order, intra-species, inter-specific, inter-genus, inter-familiar, and inter-order. We confirmed the addition of

Indexed as

cytochrome c oxidase 1DNA barcodephylogenetic treereference libraryshark and rayspecies identification

Identifiers

PMID36978544
PMCPMC10044685

What OpenQuestion holds

Textfull text, public
LicenceCC BY
measurements read40
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.