ArticleScientific reports2023
Development of loop-mediated isothermal amplification (LAMP) assays using five primers reduces the false-positive rate in COVID-19 diagnosis.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.
- Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Bacterial Bovine Mastitis: A Systematic Review.Veterinary medicine and science · 2026Pooled it
- The Application of Reverse Transcription Loop-mediated Isothermal Amplification to Detect Cell-Free Seminal mRNA.Molecular biotechnology · 2026Article
- A Rapid and Sensitive LAMP Assay for the Detection ofFoods (Basel, Switzerland) · 2026Article
- Article
- Development and validation of a species-specific loop-mediated isothermal amplification assay for rapid detection of Perkinsus marinus.Parasites, hosts and diseases · 2026Article
- Differential Capacitance Spectroscopy for Real-Time Monitoring of RNA Amplification.The journal of physical chemistry. B · 2025Article
- Design of New Primer Sets for the Development of a Loop-Mediated Isothermal Amplification for Rapid Detection ofCurrent issues in molecular biology · 2025Article
- Loop-Mediated Isothermal Amplification Assay for Visual Detection ofFoods (Basel, Switzerland) · 2025Article
- From Tradition to Innovation: Diverse Molecular Techniques in the Fight Against Infectious Diseases.Diagnostics (Basel, Switzerland) · 2024Review
- Rapid In-Field Detection of Airborne Pathogens Using Loop-Mediated Isothermal Amplification (LAMP).Microorganisms · 2024Article
- Enhanced Specificity in Colorimetric LAMP Assay forJournal of fungi (Basel, Switzerland) · 2024Article
- Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a.Animals : an open access journal from MDPI · 2024Article
- Development of a rapid LFA test based on direct RT-LAMP for diagnosis of SARS-CoV-2.Practical laboratory medicine · 2024Article
- Current advances in Hepatitis C diagnostics.Journal of biological engineering · 2024Review
- Ultra-sensitive colorimetric detection of SARS-CoV-2 by novel gold nanoparticle (AuNP)-assisted loop-mediated isothermal amplification (LAMP) and freezing methods.Mikrochimica acta · 2024Article
- Advancements in SARS-CoV-2 detection: Navigating the molecular landscape and diagnostic technologies.Heliyon · 2024Review
- A dual RPA-LFD assay for the simultaneous detection ofFrontiers in bioengineering and biotechnology · 2024Article
- Understanding theFrontiers in parasitology · 2024Review
- Detection of feline immunodeficiency virus by neutral red-based loop-mediated isothermal amplification assay.Veterinary world · 2024Article
- Evaluation of COVID-19 rapid antigen test against polymerase chain reaction test in immunocompromised patients.PloS one · 2024Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is a cheaper and faster testing alternative for detecting SARS-CoV-2. However, a high false-positive rate due to misamplification is one of the major limitations. To overcome misamplifications, we developed colorimetric and fluorometric RT-LAMP assays using five LAMP primers, instead of six. The gold-standard RT-PCR technique verified the assays' performance. Compared to other primer sets with six primers (N, S, and RdRp), the E-ID1 primer set, including five primers, performed superbly on both colorimetric and fluorometric assays. The sensitivity of colorimetric and fluorometric assays was 89.5% and 92.2%, respectively, with a limit of detection of 20 copies/µL. The colorimetric RT-LAMP had a specificity of 97.2% and an accuracy of 94.5%, while the fluorometric RT-LAMP obtained 99% and 96.7%, respectively. No misamplification was evident even after 120 min, which is crucial for the success of this technique. These findings are important to support the use of RT-LAMP in the healthcare systems in fighting COVID-19.
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