Evidence map›Paper›PMID 36976356›Full record

ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2023

A rabbit model for outer retinal atrophy caused by surgical RPE removal.

Sami Al-Nawaiseh, Christina Krötz, Annekatrin Rickmann, Claudine Strack, Anja Germann, Hagen von Briesen, Peter Szurman, André Schulz, Boris V Stanzel

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Sami Al-Nawaiseh *Eye Clinic Sulzbach, Knappschaft Hospital Saar, Sulzbach/Saar, Germany.
Christina Krötz *Fraunhofer Institute for Biomedical Engineering, Sulzbach/Saar, Germany.
Annekatrin RickmannEye Clinic Sulzbach, Knappschaft Hospital Saar, Sulzbach/Saar, Germany.
Claudine StrackDepartment of Ophthalmology, University of Bonn, Bonn, Germany.
Anja GermannFraunhofer Institute for Biomedical Engineering, Sulzbach/Saar, Germany.
Hagen von BriesenFraunhofer Institute for Biomedical Engineering, Sulzbach/Saar, Germany.
Peter SzurmanEye Clinic Sulzbach, Knappschaft Hospital Saar, Sulzbach/Saar, Germany.
André SchulzEye Clinic Sulzbach, Knappschaft Hospital Saar, Sulzbach/Saar, Germany.
Boris V StanzelEye Clinic Sulzbach, Knappschaft Hospital Saar, Sulzbach/Saar, Germany. boris.stanzel@kksaar.de.ORCID http://orcid.org/0000-0002-4316-1539
Knappschaftsklinikum Saar · DEFraunhofer Institute for Biomedical Engineering · DEUniversity of Bonn · DE

Funding

Bundesministerium für Bildung und Forschung 01EK1613ADeutsche Forschungsgemeinschaft STA 1135/2-1
6 · The paper itself

Abstract

purposeWe aimed to establish a rabbit model with retinal atrophy induced by an iatrogenic retinal pigment epithelium (RPE) removal, for future testing of the efficacy and safety of cell therapy strategies.

methodsA localized detachment of the retina from the RPE/choroid layer was created in 18 pigmented rabbits. The RPE was removed by scraping with a custom-made extendable loop instrument. The resulting RPE wound was observed over a time course of 12 weeks with optical coherence tomography and angiography. After 4 days (group 1) and 12 weeks (group 2), histology was done and staining with hematoxylin and eosin, as well as immunofluorescence performed to further investigate the effects of debridement on the RPE and the overlying retina.

resultsAlready after 4 days, we observed a closure of the RPE wound by proliferating RPE and microglia/macrophage cells forming a multilayered clump. This pattern continued over the observation time course of 12 weeks, whereby the inner and outer nuclear layer of the retina became atrophic. No neovascularization was observed in the angiograms or histology. The observed changes were limited to the site of the former RPE wound.

conclusionsLocalized surgical RPE removal induced an adjacent progressive retinal atrophy. Altering the natural course of this model may serve as a basis to test RPE cell therapeutics.

Indexed as

Retinal DegenerationRetinal Pigment EpitheliumAnimalsAtrophyChoroidFluorescein AngiographyRabbitsRetinaTomography, Optical CoherenceAnimal modelCell therapyMechanical debridementRabbitRPE degenerationSubretinal surgery

Identifiers

PMID36976356
PMCPMC10368565
OpenAlexW4361191021

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.