Evidence map›Paper›PMID 36976063›Full record

ArticleJournal of functional biomaterials2023

Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels.

Gabriela Pacheco de Almeida Braga, Karina Sampaio Caiaffa, Rafaela Laruzo Rabelo, Vanessa Rodrigues Dos Santos, Amanda Caselato Andolfatto Souza, Lucas da Silva Ribeiro, Emerson Rodrigues de Camargo, Anuradha Prakki, Cristiane Duque

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of functional biomaterials, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Gabriela Pacheco de Almeida BragaDepartment of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
Karina Sampaio CaiaffaDepartment of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
Rafaela Laruzo RabeloDepartment of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
Vanessa Rodrigues Dos SantosDepartment of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.ORCID 0000-0001-8725-2136
Amanda Caselato Andolfatto SouzaDepartment of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
Lucas da Silva RibeiroDepartment of Chemistry, Federal University of São Carlos (UFSCar), São Carlos 13565-905, SP, Brazil.
Emerson Rodrigues de CamargoDepartment of Chemistry, Federal University of São Carlos (UFSCar), São Carlos 13565-905, SP, Brazil.
Anuradha PrakkiDental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada.ORCID 0000-0002-8969-9043
Cristiane DuqueDepartment of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.ORCID 0000-0002-2575-279X
Universidade Estadual Paulista (Unesp) · BRUniversidade Federal de São Carlos · BRUniversity of Toronto · CA

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior #001Natural Sciences and Engineering Research Council 2018-06489São Paulo Research Foundation 2017/10940-1São Paulo Research Foundation 2018/12871-0
6 · The paper itself

Abstract

This study aimed to evaluate the effects of flavonoids incorporated into poly(N-vinylcaprolactam) (PNVCL) hydrogel on cell viability and mineralization markers of odontoblast-like cells. MDPC-23 cells were exposed to ampelopsin (AMP), isoquercitrin (ISO), rutin (RUT) and control calcium hydroxide (CH) for evaluation of cell viability, total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition by colorimetric assays. Based on an initial screening, AMP and CH were loaded into PNVCL hydrogels and had their cytotoxicity and effect on mineralization markers determined. Cell viability was above 70% when MDPC-23 cells were treated with AMP, ISO and RUT. AMP showed the highest ALP activity and mineralized nodule deposition. Extracts of PNVCL+AMP and PNVCL+CH in culture medium (at the dilutions of 1/16 and 1/32) did not affect cell viability and stimulated ALP activity and mineralized nodules' deposition, which were statistically higher than the control in osteogenic medium. In conclusion, AMP and AMP-loaded PNVCL hydrogels were cytocompatible and able to induce bio-mineralization markers in odontoblast-cells.

Indexed as

alkaline phosphatasecytotoxicitydentin mineralizationflavonoidshydrogelodontoblasts

Identifiers

PMID36976063
PMCPMC10058549
OpenAlexW4322771724

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.