ArticleDevelopment (Cambridge, England)2023
Next-generation plasmids for transgenesis in zebrafish and beyond.
Article in Development (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A transgenic zebrafish for spatiotemporal optogenetic activation of FGF/ERK signaling.Biology open · 2026Article
- An optogenetic toolkit for robust activation of FGF, BMP, & Nodal signaling in zebrafish.Developmental biology · 2026Article
- A transgenic zebrafish for direct optogenetic activation of FGF/ERK signaling.bioRxiv : the preprint server for biology · 2026Article
- Cp36 serine recombinase as a new tool for zebrafish transgenesis.bioRxiv : the preprint server for biology · 2026Article
- Single-cell chromatin profiling reveals dynamic regulatory logic and enhancer elements in brain and retina development.bioRxiv : the preprint server for biology · 2026Article
- Zebrafish macrophages convert physical wound signals into rapid vascular permeabilization.Nature communications · 2026Article
- Functional architecture of cardiac TF regulatory landscapes in control of mammalian heart development.bioRxiv : the preprint server for biology · 2025Article
- Developmental regulation of intestinalbioRxiv : the preprint server for biology · 2025Article
- MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox for use in vertebrate model systems.Development (Cambridge, England) · 2025Article
- Development of cell labeling and gene editing tools in urochordateMarine life science & technology · 2025Article
- The pericardium forms as a distinct structure during heart formation.Nature communications · 2025Article
- TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation.Cell reports methods · 2025Article
- The zebrafish (NAR genomics and bioinformatics · 2025Article
- Neurological Disorders Induced by Drug Use: Effects of Adolescent and Embryonic Drug Exposure on Behavioral Neurodevelopment.International journal of molecular sciences · 2024Review
- Article
- Diving into the zebrafish brain: exploring neuroscience frontiers with genetic tools, imaging techniques, and behavioral insights.Frontiers in molecular neuroscience · 2024Review
- A robust knock-in approach using a minimal promoter and a minicircle.Developmental biology · 2024Article
- Article
- Review
- Conserved enhancers control notochord expression of vertebrate Brachyury.Nature communications · 2023Article
Corrections and comments
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Authors and funding
22 authors.
Funding
Abstract
Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis paired with Gateway-compatible vector collections has transformed zebrafish transgenesis with an accessible modular system. Here, we establish several next-generation transgenesis tools for zebrafish and other species to expand and enhance transgenic applications. To facilitate gene regulatory element testing, we generated Gateway middle entry vectors harboring the small mouse beta-globin minimal promoter coupled to several fluorophores, CreERT2 and Gal4. To extend the color spectrum for transgenic applications, we established middle entry vectors encoding the bright, blue-fluorescent protein mCerulean and mApple as an alternative red fluorophore. We present a series of p2A peptide-based 3' vectors with different fluorophores and subcellular localizations to co-label cells expressing proteins of interest. Finally, we established Tol2 destination vectors carrying the zebrafish exorh promoter driving different fluorophores as a pineal gland-specific transgenesis marker that is active before hatching and through adulthood. exorh-based reporters and transgenesis markers also drive specific pineal gland expression in the eye-less cavefish (Astyanax). Together, our vectors provide versatile reagents for transgenesis applications in zebrafish, cavefish and other models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.