ArticleBMC genomics2023
Single-cell transcriptome analysis and in vitro differentiation of testicular cells reveal novel insights into male sterility of the interspecific hybrid cattle-yak.
Article in BMC genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Transcriptomic Dynamics and ECM Remodeling During Postnatal Testicular Maturation in the Tianzhu White Yak.Animals : an open access journal from MDPI · 2026Article
- scRNA-seq reveals different cell clusters in the testes of Mongolian cattle and EGR1/FOS/JUN regulation in Sertoli cells.Scientific reports · 2026Article
- Divergent Amplification of Y-Linked Dosage-Sensitive Genes Triggers Regulatory Mismatch Underlying Cattle-Yak Male Sterility.Biomolecules · 2026Article
- Single-nucleus RNA sequencing provides insights into the genetic mechanisms underlying reproductive adaptability in Tibetan sheep (Ovis aries).Communications biology · 2026Article
- Dynamic regulation of spermatogenesis and hybrid sterility revealed by single-cell analysis in yak and cattle.Molecular biology and evolution · 2026Article
- Hybridization and gene expression: Beyond differentially expressed genes.Molecular ecology · 2025Review
- Comparative Analysis of Testicular Transcriptional and Translational Landscapes in Yak and Cattle-Yak: Implications for Hybrid Male Sterility.Biomolecules · 2025Article
- Comparison of reproductive performance and functional analysis of spermatogenesis factors between domestic yak and semi-wild blood yak.BMC genomics · 2025Article
- Single-cell multiomic comparison of mouse and rat spermatogenesis reveals gene regulatory networks conserved for over 20 million years.Stem cell reports · 2025Article
- Different complex regulatory phenotypes underlie hybrid male sterility in divergent rodent crosses.Genetics · 2025Article
- Different complex regulatory phenotypes underlie hybrid male sterility in divergent rodent crosses.bioRxiv : the preprint server for biology · 2024Article
- NLife (Basel, Switzerland) · 2024Article
- Advances in single-cell transcriptomics in animal research.Journal of animal science and biotechnology · 2024Review
- Single-Nucleus RNA-Seq Reveals Spermatogonial Stem Cell Developmental Pattern in Shaziling Pigs.Biomolecules · 2024Article
- mRNA-Seq of testis and liver tissues reveals a testis-specific gene and alternative splicing associated with hybrid male sterility in dzo.Journal of animal science · 2024Article
Corrections and comments
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Authors and funding
18 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundInterspecific hybridization plays vital roles in enriching animal diversity, while male hybrid sterility (MHS) of the offspring commonly suffered from spermatogenic arrest constitutes the postzygotic reproductive isolation. Cattle-yak, the hybrid offspring of cattle (Bos taurus) and yak (Bos grunniens) can serve as an ideal MHS animal model. Although meiotic arrest was found to contribute to MHS of cattle-yak, yet the cellular characteristics and developmental potentials of male germline cell in pubertal cattle-yak remain to be systematically investigated.
resultsSingle-cell RNA-seq analysis of germline and niche cell types in pubertal testis of cattle-yak and yak indicated that dynamic gene expression of developmental germ cells was terminated at late primary spermatocyte (meiotic arrest) and abnormal components of niche cell in pubertal cattle-yak. Further in vitro proliferation and differentially expressed gene (DEG) analysis of specific type of cells revealed that undifferentiated spermatogonia of cattle-yak exhibited defects in viability and proliferation/differentiation potentials.
conclusionComparative scRNA-seq and in vitro proliferation analysis of testicular cells indicated that not only meiotic arrest contributed to MHS of cattle-yak. Spermatogenic arrest of cattle-yak may originate from the differentiation stage of undifferentiated spermatogonia and niche cells of cattle-yak may provide an adverse microenvironment for spermatogenesis.
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