Evidence map›Paper›PMID 36973590›Full record

ArticleNeurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology2023

Coordinated modification in expression levels of HSPA1A/B, DGKH, and NOTCH2 in Parkinson's patients' blood and substantia nigra as a diagnostic sign: the transcriptomes' relationship.

Leila Asad Samani, Kamran Ghaedi, Ahmad Majd, Maryam Peymani, Masoud Etemadifar

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Article in Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

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0cells of the map it votes in
9citing papers in PubMed
7.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
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  7. An Overview of Epigenetic Changes in the Parkinson's Disease Brain.International journal of molecular sciences · 2024
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4 · The record

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5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Leila Asad SamaniDepartment of Cellular and Molecular Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Kamran GhaediDepartment of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Azadi Sq., Hezar Jerib Ave, P.O. Code, Isfahan, 81746-73441, Iran. kamranghaedi@sci.ui.ac.ir.ORCID http://orcid.org/0000-0002-9790-5644
Ahmad MajdDepartment of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran. a_majd@iau-tnb.ac.ir.
Maryam PeymaniDepartment of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Masoud EtemadifarDepartment of Neurology and Isfahan Neurosurgery Research Center, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Islamic Azad University North Tehran Branch · IRIsfahan University of Medical Sciences · IRIsfahan University of Technology · IRIslamic Azad University of Shahrekord · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDiagnosis of Parkinson's disease (PD) is associated with a vast number of challenges. This study aimed to assess the overlap of PD patients' transcriptomes in the substantia nigra (SN) with peripheral blood mononuclear cells (PBMCs) to discover potential biomarkers for diagnosis.

methodsGEO data were used to select genes with significant changes in expression level in the SN region and eligible studies. Also, transcriptome data related to blood of PD patients with other neurodegenerative diseases (ND) was considered. Differential expression genes between PD and control were evaluated in the SN and blood, and RT-qPCR was applied to validate the findings.

resultsAt the expression level, no significant similarity in long non-coding RNA was found between the patients' SN and blood. While in silico results revealed 16 common mRNAs in SN and blood with significant expression levels. Among all overexpressed mRNAs, HSPA1A/B expression level had the highest expression difference between control and PD samples. Moreover, DGKH had the highest score of down-regulated genes in both blood and SN. The NOTCH pathway had the highest score pathway among up-regulated pathways, and the expression levels of NOTCH2, H4C8, and H2BC21 associated with this pathway had the most ability to separate the control and PD populations. Furthermore, RT-qPCR results revealed that HSPA1A/B, NOTCH2, and H4C8 were overexpressed in PD PBMCs, while DGKH expression levels were lower compared to controls.

conclusionOur findings indicate that expression levels of HSPA1A/B, DGKH, and NOTCH2 could be applied as candidate biomarkers to diagnose PD patients in the SN region and PBMCs.

Indexed as

Parkinson DiseaseTranscriptomeBiomarkersHSP70 Heat-Shock ProteinsHumansLeukocytes, MononuclearReceptor, Notch2Substantia NigraBiomarkersHSP70 Heat-Shock ProteinsHSPA1A protein, humanNOTCH2 protein, humanReceptor, Notch2BiomarkerGene expressionMicroarrayNeurodegenerative diseases

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PMID36973590
OpenAlexW4360989822

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.