ArticleNature methods2023
High-speed low-light in vivo two-photon voltage imaging of large neuronal populations.
Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 102 citations in OpenAlex.
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- Light-addressable potentiometric sensors: principles, strategies, and applications.Chemical communications (Cambridge, England) · 2026Review
- Sub-millivolt voltage imaging reveals gap junction-mediated bioelectric contact inhibition.Nature communications · 2026Article
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- A versatile platform for two-photon neuronal population voltage imaging across cortical depths.Nature methods · 2026Article
- FACED 2.0 enables large-scale voltage and calcium imaging in vivo.Nature methods · 2026Article
- Three-dimensional voltage imaging in live larval zebrafish brains using fully genetically encoded voltage indicator.Scientific reports · 2026Article
- Precise calcium-to-spike inference using biophysical generative models.bioRxiv : the preprint server for biology · 2026Article
- A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo.bioRxiv : the preprint server for biology · 2026Article
- A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo.bioRxiv : the preprint server for biology · 2026Article
- Voltage imaging in zebrafish using high-speed light-sheet microscopy.Neurophotonics · 2026Review
- Voltage imaging as a window into neural computation.Neurophotonics · 2026Article
- Article
- Scattering-enabled epi-quantitative phase imaging reveals subcellular detail in organoids and deep mouse brains.bioRxiv : the preprint server for biology · 2026Article
- Voltage imaging with periodic structured illumination.Biomedical optics express · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- High-speed neural imaging with multiplexed miniaturized two-photon microscopy.Cell reports methods · 2025Article
- Dual-Channel Event Microscopy for Ultrafast Biological Imaging.bioRxiv : the preprint server for biology · 2025Article
- Real-time self-supervised denoising for high-speed fluorescence neural imaging.Nature communications · 2025Article
- All-optical voltage interrogation for probing synaptic plasticity in vivo.Nature communications · 2025Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Monitoring spiking activity across large neuronal populations at behaviorally relevant timescales is critical for understanding neural circuit function. Unlike calcium imaging, voltage imaging requires kilohertz sampling rates that reduce fluorescence detection to near shot-noise levels. High-photon flux excitation can overcome photon-limited shot noise, but photobleaching and photodamage restrict the number and duration of simultaneously imaged neurons. We investigated an alternative approach aimed at low two-photon flux, which is voltage imaging below the shot-noise limit. This framework involved developing positive-going voltage indicators with improved spike detection (SpikeyGi and SpikeyGi2); a two-photon microscope ('SMURF') for kilohertz frame rate imaging across a 0.4 mm × 0.4 mm field of view; and a self-supervised denoising algorithm (DeepVID) for inferring fluorescence from shot-noise-limited signals. Through these combined advances, we achieved simultaneous high-speed deep-tissue imaging of more than 100 densely labeled neurons over 1 hour in awake behaving mice. This demonstrates a scalable approach for voltage imaging across increasing neuronal populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.