ArticleNature plants2023
Multi-Knock-a multi-targeted genome-scale CRISPR toolbox to overcome functional redundancy in plants.
Article in Nature plants, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 62 citations in OpenAlex.
- Purine permease 5 contributes to riboflavin distribution in Arabidopsis reproductive organs.Plant & cell physiology · 2026Article
- The BAHD Acyltransferase Gene Family: Evolutionary Dynamics, Biochemical Mechanisms, and Roles in Plant Stress Adaptation.Plant biotechnology journal · 2026Review
- Targeting redundant gene families: A multiplexed, tissue-specific CRISPR toolbox for Arabidopsis genetic screens.Cell reports · 2026Article
- mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis.Plant physiology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Beyond efficiency: The multi-scale architecture of robust water transport in plants.Quantitative plant biology · 2026Review
- PLETHORAs shape Arabidopsis phyllotaxis through modulation of patterning robustness and accelerated inflorescence development.The New phytologist · 2026Article
- A CRISPR-Cas9 library to target putative redundant gene sets facilitates their functional exploration in grain development in rice.BMC plant biology · 2025Article
- Hazelnut allergome overview and Cor a gRNAs identification.BMC plant biology · 2025Article
- Jack of all trades: crosstalk between FERONIA signaling and hormone pathways.Journal of experimental botany · 2025Review
- Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level.Nature communications · 2025Article
- Structural basis of cytokinin transport by the Arabidopsis AZG2.Nature communications · 2025Article
- Advances in lignocellulosic feedstocks for bioenergy and bioproducts.Nature communications · 2025Review
- Decoding Plant-Pathogen Interactions: A Comprehensive Exploration of Effector-Plant Transcription Factor Dynamics.Molecular plant pathology · 2025Review
- The cytokinin efflux transporter ABCC4 participates in Arabidopsis root system development.Plant physiology · 2024Article
- Exploring the function of plant root diffusion barriers in sealing and shielding for environmental adaptation.Nature plants · 2024Review
- Reporter Alleles in hiPSCs: Visual Cues on Development and Disease.International journal of molecular sciences · 2024Review
- An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus.Plant methods · 2024Article
- Heat Stress and Plant-Biotic Interactions: Advances and Perspectives.Plants (Basel, Switzerland) · 2024Review
- Spatially distributed cytokinins: Metabolism, signaling, and transport.Plant communications · 2024Review
Corrections and comments
- Commented on by
- Commented on byA ton of Plants.2023
Authors and funding
21 authors at 4 institutions in 4 countries.
Funding
Abstract
Plant genomes are characterized by large and complex gene families that often result in similar and partially overlapping functions. This genetic redundancy severely hampers current efforts to uncover novel phenotypes, delaying basic genetic research and breeding programmes. Here we describe the development and validation of Multi-Knock, a genome-scale clustered regularly interspaced short palindromic repeat toolbox that overcomes functional redundancy in Arabidopsis by simultaneously targeting multiple gene-family members, thus identifying genetically hidden components. We computationally designed 59,129 optimal single-guide RNAs that each target two to ten genes within a family at once. Furthermore, partitioning the library into ten sublibraries directed towards a different functional group allows flexible and targeted genetic screens. From the 5,635 single-guide RNAs targeting the plant transportome, we generated over 3,500 independent Arabidopsis lines that allowed us to identify and characterize the first known cytokinin tonoplast-localized transporters in plants. With the ability to overcome functional redundancy in plants at the genome-scale level, the developed strategy can be readily deployed by scientists and breeders for basic research and to expedite breeding efforts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.