Evidence map›Paper›PMID 36972428›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2023

A general mechanism for transcription bubble nucleation in bacteria.

Andreas U Mueller, James Chen, Mengyu Wu, Courtney Chiu, B Tracy Nixon, Elizabeth A Campbell, Seth A Darst

Open access · greenAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Real-time capture of σNature communications · 2025
    Article
  3. Article
  4. Transcription activation inEcoSal Plus · 2024
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Andreas U MuellerLaboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065.ORCID 0000-0003-3002-3695
James ChenLaboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065.ORCID 0000-0002-2311-003X
Mengyu WuLaboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065.ORCID 0000-0002-2248-9383
Courtney ChiuLaboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065.
B Tracy NixonDepartment of Biochemistry and Molecular Biology, Penn State University, University Park, PA 16802.ORCID 0000-0002-3568-5703
Elizabeth A CampbellLaboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065.ORCID 0000-0002-1332-128X
Seth A DarstLaboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065.ORCID 0000-0002-8241-3153
Rockefeller University · USPennsylvania State University · US

Funding

TRD #3: An automated and streamlined pipeline for in-situ molecular microscopyP41GM103310 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2012 to 2021
$14.7M
Structure, function, and regulation of the bacterial transcription cycleR35GM118130 · NIGMS · ROCKEFELLER UNIVERSITY · PI DARST, SETH A. · 2016 to 2025
$8.9M
Acquisition of Detector for JEOL 3200 FSC Electron MicroscopeS10OD019994 · OD · NEW YORK STRUCTURAL BIOLOGY CENTER · PI CARRAGHER, BRIDGET OLIVIA · 2015 to 2015
$600k
NIGMS NIH HHS P41 GM103310NIGMS NIH HHS R35 GM118130NIH HHS S10 OD019994
6 · The paper itself

Abstract

Bacterial transcription initiation requires σ factors for nucleation of the transcription bubble. The canonical housekeeping σ factor, σ

Indexed as

Escherichia coliTranscription Initiation, GeneticAquifexCryoelectron MicroscopyDNA-Directed RNA PolymerasesEscherichia coli ProteinsPromoter Regions, GeneticRNA Polymerase Sigma 54Sigma FactorDNA-Directed RNA PolymerasesEscherichia coli ProteinsRNA Polymerase Sigma 54RNA polymerase sigma 70rpoN protein, E coliSigma Factorbacterial transcriptionRpoNsigma 54transcription bubble nucleation

Identifiers

PMID36972428
PMCPMC10083551
OpenAlexW4360992881

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.