Evidence map›Paper›PMID 36972300›Full record

ArticleACS synthetic biology2023

Optimization of Campesterol-Producing Yeast Strains as a Feasible Platform for the Functional Reconstitution of Plant Membrane-Bound Enzymes.

Shanhui Xu, Xiaoxuan Teng, Yanran Li

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Shanhui XuDepartment of Chemical and Environmental Engineering, University of California, 900 University Avenue, Bourns Hall, Suite A220, Riverside, California 92521, United States.
Xiaoxuan TengDepartment of Chemical and Environmental Engineering, University of California, 900 University Avenue, Bourns Hall, Suite A220, Riverside, California 92521, United States.ORCID 0000-0001-9568-027X
Yanran LiDepartment of Chemical and Environmental Engineering, University of California, 900 University Avenue, Bourns Hall, Suite A220, Riverside, California 92521, United States.ORCID 0000-0001-8709-3497
University of California, Riverside · US

Funding

Reprogramming Plant Perception for Novel Plant Natural Products DiscoveryDP2AT011445 · NCCIH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LI, YANRAN · 2020 to 2020
$2.3M
NCCIH NIH HHS DP2 AT011445
6 · The paper itself

Abstract

Campesterol is a major phytosterol that plays important roles in regulating membrane properties and serves as the precursor to multiple specialized metabolites, such as the phytohormone brassinosteroids. Recently, we established a campesterol-producing yeast strain and extended the bioproduction to 22-hydroxycampesterol and 22-hydroxycampest-4-en-3-one, the precursors to brassinolide. However, there is a trade-off in growth due to the disrupted sterol metabolism. In this study, we enhanced the growth of the campesterol-producing yeast by partially restoring the activity of the sterol acyltransferase and engineering upstream FPP supply. Furthermore, genome sequencing analysis also revealed a pool of genes possibly associated with the altered sterol metabolism. Retro engineering implies an essential role of ASG1, especially the C-terminal asparagine-rich domain of ASG1, in the sterol metabolism of yeast especially under stress. The performance of the campesterol-producing yeast strain was enhanced with the titer of campesterol to 18.4 mg/L, and the stationary OD

Indexed as

PhytosterolsSaccharomyces cerevisiaeCholesterolPlant ProteinscampesterolCholesterolPhytosterolsPlant Proteinscampesterolgenome-scale screeningmembrane-bound enzymesmetabolic engineeringyeast

Identifiers

PMID36972300
PMCPMC11531777
OpenAlexW4361017347

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.