Evidence map›Paper›PMID 36971858›Full record

ArticleMikrochimica acta2023

Determination of trypsin using protamine mediated fluorescent enhancement of DNA templated Au nanoclusters.

Ning-Ning Wu, Lin-Ge Chen, Min-Zhi Xiao, Rong-Yao Yuan, Hai-Bo Wang

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
3.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 41 citations in OpenAlex.

  1. Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Ning-Ning WuCollege of Chemistry and Chemical Engineering, Xinyang Key Laboratory of Functional Nanomaterials for Bioanalysis, Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, 464000, People's Republic of China.
Lin-Ge ChenCollege of Chemistry and Chemical Engineering, Xinyang Key Laboratory of Functional Nanomaterials for Bioanalysis, Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, 464000, People's Republic of China.
Min-Zhi XiaoSchool of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha, 410114, People's Republic of China.
Rong-Yao YuanSchool of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha, 410114, People's Republic of China.
Hai-Bo WangCollege of Chemistry and Chemical Engineering, Xinyang Key Laboratory of Functional Nanomaterials for Bioanalysis, Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, 464000, People's Republic of China. wanghaibohn@163.com.ORCID http://orcid.org/0000-0002-8064-1400
Xinyang Normal University · CNChangsha University of Science and Technology · CN

Funding

National Natural Science Foundation of China U1704153
6 · The paper itself

Abstract

A fluorescent method is described for trypsin determination through the strong electrostatic interactions between cationic polyelectrolytes and single-stranded DNA (ssDNA) templated Au nanoclusters (AuNCs). The ssDNA-AuNCs display improved fluorescence emission with excitation/emission maxima at 280/475 nm after being incorporated with poly(diallyldimethylammonium chloride) (PDDA). Fluorescent enhancement is mainly attributed to the electrostatic interactions occurring  between PDDA and ssDNA templates. This can make the conformation of the ssDNA templates to change. Thus, it offers a better microenvironment for stabilizing and protecting ssDNA-AuNCs, and results in fluorescence emission enhancement. By using protamine as a model, the method is employed for the determination of trypsin. The assay enables trypsin to be determined with good sensitivity and a linear response ranging from 5 ng⋅mL

Indexed as

Metal NanoparticlesDNA, Single-StrandedFluorescent DyesGoldHumansProtaminesTrypsinDNA, Single-StrandedFluorescent DyesGoldProtaminesTrypsinCationic polyelectrolytesConformation changesElectrostatic interactionsFluorescent method

Identifiers

PMID36971858
OpenAlexW4361017360

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.