Evidence map›Paper›PMID 36966253›Full record

ArticleCommunications biology2023

IKBA phosphorylation governs human sperm motility through ACC-mediated fatty acid beta-oxidation.

Yanquan Li, Youwei Hu, Zhengquan Wang, Tingting Lu, Yiting Yang, Hua Diao, Xiaoguo Zheng, Chong Xie, Ping Zhang, Xuelian Zhang and 1 more

Open access · goldFull text read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Yanquan LiInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Youwei HuState Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, China.
Zhengquan WangInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Tingting LuInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Yiting YangNPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute for Biomedical and Pharmaceutical Technologies, 200032, Shanghai, China.
Hua DiaoNPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute for Biomedical and Pharmaceutical Technologies, 200032, Shanghai, China.
Xiaoguo ZhengInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Chong XieInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Ping ZhangInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. shping1216@163.com.ORCID 0000-0001-8364-2444
Xuelian ZhangState Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, China. xuelianzhang@fudan.edu.cn.ORCID 0000-0002-4999-3598
Yuchuan ZhouInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. zhouych@sibcb.ac.cn.ORCID 0000-0002-9501-5568
Shanghai Jiao Tong University · CNFudan University · CNShanghai CASB Biotechnology (China) · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The nuclear factor-κB (NF-κB) signaling pathway regulates specific immunological responses and controls a wide range of physiological processes. NF-κB inhibitor alpha (IKBA) is an NF-κB inhibitory mediator in the cytoplasm that modulates the nuclear translocation and DNA binding activities of NF-κB proteins. However, whether the upstream cascade of the canonical NF-κB signaling pathway has physiological roles independent of IKBA-mediated transcriptional activation remains unclear. Herein we investigated the function of IKBA in mature sperm in which transcriptional and translational events do not occur. IKBA was highly expressed in human sperm. The repression of IKBA phosphorylation by its inhibitor Bay117082 markedly enhanced sperm motility. On the contrary, lipopolysaccharide-stimulated IKBA phosphorylation significantly decreased sperm motility. Nevertheless, Bay117082 treatment did not affect the motility of IKBA-knockout sperm. Further, untargeted metabolomic analysis and pharmacological blocking assays revealed that the Bay117082-induced increase in sperm motility was attributable to fatty acid β-oxidation (FAO) enhancement. In addition, we found that IKBA phosphorylation inhibition resulted in a significant reduction of acetyl-CoA carboxylase levels in the FAO metabolic pathway. Our findings indicate that IKBA-mediated signaling orchestrates sperm motility program and improves our understanding of transcription-independent NF-κB signaling pathway in cells.

Indexed as

NF-kappa BNF-KappaB Inhibitor alphaSperm MotilityFatty AcidsHumansMaleNitrilesPhosphorylationSemenSulfones3-(4-methylphenylsulfonyl)-2-propenenitrileFatty AcidsNF-kappa BNF-KappaB Inhibitor alphaNFKBIA protein, humanNitrilesSulfones

Identifiers

PMID36966253
PMCPMC10039860
OpenAlexW4360950025

What OpenQuestion holds

Textfull text, public
LicenceCC BY
measurements read42
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.