Evidence map›Paper›PMID 36966053›Full record

ArticleJournal of bioscience and bioengineering2023

Microfluidic encapsulation of photosynthetic cyanobacteria in hydrogel microparticles augments oxygen delivery to rescue ischemic myocardium.

Lyndsay Mariah Stapleton, Justin Michael Farry, Yuanjia Zhu, Haley Joan Lucian, Hanjay Wang, Michael John Paulsen, Kailey Pearl Totherow, Gillie Agmon Roth, Kara Kimberly Brower, Polly Morrell Fordyce and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of bioscience and bioengineering, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Towards chlorocytes for therapeutic intravascular photosynthesis.Applied microbiology and biotechnology · 2024
    Review
  5. Review
  6. Review
  7. Review
  8. Engineering Organ-on-a-Chip Systems for Vascular Diseases.Arteriosclerosis, thrombosis, and vascular biology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Lyndsay Mariah StapletonDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Cardiothoracic Surgery, Stanford University, Stanford, CA 94305, USA.
Justin Michael FarryDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA 94305, USA.
Yuanjia ZhuDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Cardiothoracic Surgery, Stanford University, Stanford, CA 94305, USA.
Haley Joan LucianDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA 94305, USA.
Hanjay WangDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA 94305, USA.
Michael John PaulsenDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA 94305, USA.
Kailey Pearl TotherowDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Gillie Agmon RothDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Kara Kimberly BrowerDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Polly Morrell FordyceDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA; ChEM-H Institute, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub, San Francisco, CA 94110, USA.
Eric Andrew AppelDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Materials Science & Engineering, Stanford University, Stanford, CA 94305, USA.
Yi-Ping Joseph WooDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Cardiothoracic Surgery, Stanford University, Stanford, CA 94305, USA. Electronic address: joswoo@stanford.edu.
Stanford University · USChan Zuckerberg Initiative (United States) · US

Funding

ANGIOGENIC TISSUE ENGINEERING TO LIMIT POST-INFARCTION VENTRICULAR REMODELINGR01HL089315 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI WOO, Y JOSEPH · 2008 to 2023
$6.4M
NHLBI NIH HHS R01 HL089315
6 · The paper itself

Abstract

Cardiovascular disease, primarily caused by coronary artery disease, is the leading cause of death in the United States. While standard clinical interventions have improved patient outcomes, mortality rates associated with eventual heart failure still represent a clinical challenge. Macrorevascularization techniques inadequately address the microvascular perfusion deficits that persist beyond primary and secondary interventions. In this work, we investigate a photosynthetic oxygen delivery system that rescues the myocardium following acute ischemia. Using a simple microfluidic system, we encapsulated Synechococcus elongatus into alginate hydrogel microparticles (HMPs), which photosynthetically deliver oxygen to ischemic tissue in the absence of blood flow. We demonstrate that HMPs improve the viability of S. elongatus during the injection process and allow for simple oxygen diffusion. Adult male Wistar rats (n = 45) underwent sham surgery, acute ischemia reperfusion surgery, or a chronic ischemia reperfusion surgery, followed by injection of phosphate buffered saline (PBS), S. elongatus suspended in PBS, HMPs, or S. elongatus encapsulated in HMPs. Treatment with S. elongatus-HMPs mitigated cellular apoptosis and improved left ventricular function. Thus, delivery of S. elongatus encapsulated in HMPs improves clinical translation by utilizing a minimally invasive delivery platform that improves S. elongatus viability and enhances the therapeutic benefit of a novel photosynthetic system for the treatment of myocardial ischemia.

Indexed as

CyanobacteriaHydrogelsAnimalsMaleMicrofluidicsMyocardiumOxygenRatsRats, WistarHydrogelsOxygenHydrogelIschemia reperfusionMicrofluidicsMicroparticlesOxygen delivery

Identifiers

PMID36966053
PMCPMC13003611
OpenAlexW4360612579

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.