Evidence map›Paper›PMID 36965613›Full record

ArticleActa tropica2023

Infectivity of three Mayaro Virus geographic isolates in human cell lines.

Aum R Patel, Melissa Dulcey, Nabil Abid, Melanie N Cash, Jordan Dailey, Marco Salemi, Carla Mavian, Amy Y Vittor

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta tropica, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Mayaro virus pathogenesis and immunity in rhesus macaques.PLoS neglected tropical diseases · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Aum R PatelDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, USA.
Melissa DulceyDepartment of Environmental and Global Health, University of Florida, Gainesville, FL, USA.
Nabil AbidHigh Institute of Biotechnology, University of Monastir, Tunisia; Faculty of Pharmacy of Monastir, Laboratory of Transmissible Diseases and Biological Active Substances, University of Monastir, Tunisia.
Melanie N CashDepartment of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, USA; Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA.
Jordan DaileyCollege of Medicine, Virginia Commonwealth University, VA, USA.
Marco SalemiDepartment of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, USA; Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA.
Carla MavianDepartment of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, USA; Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA. Electronic address: cmavian@ufl.edu.
Amy Y VittorEmerging Pathogens Institute, University of Florida, Gainesville, FL, USA; Division of Infectious Disease and Global Medicine, University of Florida, Gainesville, FL, USA. Electronic address: amy.vittor@medicine.ufl.edu.
University of Florida · USIcahn School of Medicine at Mount Sinai · USUniversity of Florida Health · USUniversity of Monastir · TNVirginia Commonwealth University · US

Funding

Heterologous immunity and host susceptibility to emerging alphaviral infectionsK08AI116980 · NIAID · UNIVERSITY OF FLORIDA · PI VITTOR, AMY YOMIKO · 2016 to 2020
$888k
NIAID NIH HHS K08 AI116980
6 · The paper itself

Abstract

Mayaro virus (MAYV) is an emergent arthropod-borne virus that causes an acute febrile illness accompanied by arthralgia, similar to chikungunya virus. Increasing urbanization of MAYV outbreaks in the Americas has led to concerns for geographic expansion and spillover. Given the potential importance of this pathogen, we sought to fill critical gaps in knowledge regarding MAYV infectivity and geographic variation. This study describes the cytopathogenicity of MAYV in human dermal fibroblasts, human skeletal muscle satellite cells, human embryonic kidney cells (HEK), peripherally derived human macrophages, and Vero cells. We found that regional differences between these viruses do not affect replication kinetics, with high titers peaking at 37 h post infection. MAYV-U, did however, cause the most cytopathic effect in a time-dependent manner. Compared to the other two prototypic isolates, MAYV-U harbors unique mutations in the E2 protein, D60G and S205F, that are likely to interact with the host cell receptor and could affect infectivity. We further demonstrate that pre-treatment of cells with interferon-β inhibited viral replication in a dose-dependent manner. Together, these findings advance our understanding of MAYV infection of human target cells and provide initial data regarding variation according to geography.

Indexed as

AlphavirusAlphavirus InfectionsChikungunya virusAmericasAnimalsChlorocebus aethiopsHumansVero CellsVirus ReplicationArbovirusCytopathogenicityEnvelopeGeographic variationInterferonStructure

Identifiers

PMID36965613
PMCPMC12797270
OpenAlexW4360603969

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.