Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation R521G (c.1561C > G) in FUS gene.

Masuma Akter, Haochen Cui, Md Abir Hosain, Baojin Ding

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Article in Stem cell research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.5field-weighted citation impact, top 34% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation P525L (c.1574C > T) in FUS gene.Stem cell research · 2023
Article

4 · The record

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5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Masuma AkterDepartment of Biochemistry and Molecular Biology, Louisiana State University Health Sciences, Center at Shreveport, Shreveport, LA 71130-3932, USA.

Haochen CuiDepartment of Biochemistry and Molecular Biology, Louisiana State University Health Sciences, Center at Shreveport, Shreveport, LA 71130-3932, USA.

Md Abir HosainDepartment of Biochemistry and Molecular Biology, Louisiana State University Health Sciences, Center at Shreveport, Shreveport, LA 71130-3932, USA.

Baojin DingDepartment of Biochemistry and Molecular Biology, Louisiana State University Health Sciences, Center at Shreveport, Shreveport, LA 71130-3932, USA. Electronic address: Baojin.Ding@lsuhs.edu.

Louisiana State University Health Sciences Center Shreveport · US

Funding

Determining the Pathogenesis of DYT1 Dystonia in Reprogrammed Human NeuronsR21NS112910 · NINDS · UNIVERSITY OF LOUISIANA AT LAFAYETTE · PI DING, BAOJIN · 2020 to 2020

$399k

NINDS NIH HHS R21 NS112910

6 · The paper itself

Abstract

Mutations in the RNA-binding protein FUS (fused in sarcoma) are linked to amyotrophic lateral sclerosis (ALS), but the pathogenesis is not fully understood. For modeling ALS, here we generated two induced pluripotent stem cell (iPSC) lines carrying the heterozygous and homozygous R521G (c.1561C > G) mutation in the FUS gene via genetic modification of a healthy hiPSC line (WTC11, UCSFi001-A). Both lines show normal stem cell morphology and karyotype, express pluripotent markers, and can differentiate into three germ layers, providing a valuable resource in determining the pathological mechanisms underlying the FUS mutation of R521G in ALS.

Indexed as

Amyotrophic Lateral SclerosisInduced Pluripotent Stem CellsHeterozygoteHumansKaryotypeMutationRNA-Binding Protein FUSFUS protein, humanRNA-Binding Protein FUS

Identifiers

PMID36965406

DOI10.1016/j.scr.2023.103078

PMCPMC10353567

OpenAlexW4328108616

What OpenQuestion holds

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.