ArticlePlant biotechnology journal2023
Multiplex CRISPR/Cas9-mediated mutagenesis of alfalfa FLOWERING LOCUS Ta1 (MsFTa1) leads to delayed flowering time with improved forage biomass yield and quality.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Functional analysis ofPlant signaling & behavior · 2026Article
- Overcoming breeding barriers with genome editing in autopolyploid crops.BMC plant biology · 2026Review
- Callus-mediated plant regeneration and CRISPR/Cas9-targeted mutagenesis inHorticulture research · 2026Article
- Advances in gene editing for legume improvement: technologies, progress, and prospects.Frontiers in genome editing · 2026Review
- Genome-wide unraveling of theFrontiers in plant science · 2026Article
- Development and Application of an In Vitro Pollen Viability Assay for Comparative Safety Assessment of Transgenic Alfalfa (Plants (Basel, Switzerland) · 2025Article
- Recent advances in breeding systems and their improvement in forage crops.Molecular biology reports · 2025Review
- Revolution and advances in gene editing and genomics technology for developing climate-resilient legume crops: developments and prospects.Plant molecular biology · 2025Review
- Advances in basic biology of alfalfa (Horticulture research · 2025Article
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Harnessing the Potential of CRISPR/Cas in Targeted Alfalfa Improvement for Stress Resilience.International journal of molecular sciences · 2025Review
- Manipulation of WUSCHEL orthologue expression improves the forage yield and quality in Medicago.Plant biotechnology journal · 2025Article
- Convenient, high-efficiency multiplex genome editing in autotetraploid alfalfa using endogenousaBIOTECH · 2025Article
- Advances in lignocellulosic feedstocks for bioenergy and bioproducts.Nature communications · 2025Review
- Genome-wide identification and expression analysis of CBF/DREB1 gene family in Medicago sativa L. and functional verification of MsCBF9 affecting flowering time.BMC plant biology · 2025Article
- Genome editing for grass improvement and future agriculture.Horticulture research · 2025Article
- CRISPR/Cas9-Engineering for Increased Amylolytic Potential of Microbes for Sustainable Wastewater Treatment: A Review.Current microbiology · 2024Review
- MsTFL1A delays flowering and regulates shoot architecture and root development in Medicago sativa.Plant reproduction · 2024Article
- Phospholipid Signaling in Crop Plants: A Field to Explore.Plants (Basel, Switzerland) · 2024Article
- Overexpression ofPlants (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alfalfa (Medicago sativa L.) is a perennial flowering plant in the legume family that is widely cultivated as a forage crop for its high yield, forage quality and related agricultural and economic benefits. Alfalfa is a photoperiod sensitive long-day (LD) plant that can accomplish its vegetative and reproductive phases in a short period of time. However, rapid flowering can compromise forage biomass yield and quality. Here, we attempted to delay flowering in alfalfa using multiplex CRISPR/Cas9-mediated mutagenesis of FLOWERING LOCUS Ta1 (MsFTa1), a key floral integrator and activator gene. Four guide RNAs (gRNAs) were designed and clustered in a polycistronic tRNA-gRNA system and introduced into alfalfa by Agrobacterium-mediated transformation. Ninety-six putative mutant lines were identified by gene sequencing and characterized for delayed flowering time and related desirable agronomic traits. Phenotype assessment of flowering time under LD conditions identified 22 independent mutant lines with delayed flowering compared to the control. Six independent Msfta1 lines containing mutations in all four copies of MsFTa1 accumulated significantly higher forage biomass yield, with increases of up to 78% in fresh weight and 76% in dry weight compared to controls. Depending on the harvesting schemes, many of these lines also had reduced lignin, acid detergent fibre (ADF) and neutral detergent fibre (NDF) content and significantly higher crude protein (CP) and mineral contents compared to control plants, especially in the stems. These CRISPR/Cas9-edited Msfta1 mutants could be introduced in alfalfa breeding programmes to generate elite transgene-free alfalfa cultivars with improved forage biomass yield and quality.
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