Evidence map›Paper›PMID 36963552›Full record

ArticleInternational journal of biological macromolecules2023

Structure, anti-SARS-CoV-2, and anticoagulant effects of two sulfated galactans from the red alga Botryocladia occidentalis.

Antim K Maurya, Poonam Sharma, Priyanka Samanta, Anter A Shami, Sandeep K Misra, Fuming Zhang, Reena Thara, Deepak Kumar, Deling Shi, Robert J Linhardt and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of biological macromolecules, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
4.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Antim K MauryaDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.
Poonam SharmaCenter for Immunology and Microbial Research, Department of Cell and Molecular Biology, University of Mississippi Medical Center, Jackson, MS, USA.
Priyanka SamantaDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.
Anter A ShamiDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.
Sandeep K MisraDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.
Fuming ZhangCenter for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA.
Reena TharaDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA.
Deepak KumarCenter for Immunology and Microbial Research, Department of Cell and Molecular Biology, University of Mississippi Medical Center, Jackson, MS, USA.
Deling ShiCenter for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA.
Robert J LinhardtCenter for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA.
Joshua S SharpDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA; Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, Oxford, MS, USA; Department of Chemistry and Biochemistry, University of Mississippi, Oxford, MS, USA.
Robert J DoerksenDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA; Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, Oxford, MS, USA.
Ritesh TandonDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA; Center for Immunology and Microbial Research, Department of Cell and Molecular Biology, University of Mississippi Medical Center, Jackson, MS, USA; Department of Medicine, University of Mississippi Medical Center, Jackson, MS, USA.
Vitor H PominDepartment of BioMolecular Sciences, University of Mississippi, Oxford, MS, USA; Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, Oxford, MS, USA. Electronic address: vpomin@olemiss.edu.
University of Mississippi · USRensselaer Polytechnic Institute · USUniversity of Mississippi Medical Center · USJackson Memorial Hospital · US

Funding

Tracking Glycosylation State with Fluorinated Glycosides ProbesP20GM130460 · NIGMS · UNIVERSITY OF MISSISSIPPI · PI Joshua S Sharp · 2020 to 2026
$17.9M
Initiation of immune responses to SARS COV2 in the oral cavity and upper airwayR01DE031928 · NIDCR · UNIVERSITY OF MISSISSIPPI MED CTR · PI GILL DIAMOND, SARAH C GLOVER · 2022 to 2026
$3.8M
Disruption of CS/KSPG-Mediated Neuronal Outgrowth InhibitionR03NS110996 · NINDS · UNIVERSITY OF MISSISSIPPI · PI POMIN, VITOR HUGO · 2020 to 2020
$142k
NIDCR NIH HHS R01 DE031928NIGMS NIH HHS P20 GM130460NINDS NIH HHS R03 NS110996
6 · The paper itself

Abstract

The structure of the sulfated galactan from the red alga Botryocladia occidentalis (BoSG) was originally proposed as a simple repeating disaccharide of alternating 4-linked α-galactopyranose (Galp) and 3-linked β-Galp units with variable sulfation pattern. Abundance was estimated only for the α-Galp units: one-third of 2,3-disulfation and one-third of 2-monosulfation. Here, we isolated again the same BoSG fractions from the anion-exchange chromatography, obtaining the same NMR profile of the first report. More careful NMR analysis led us to revise the structure. A more complex sulfation pattern was noted along with the occurrence of 4-linked α-3,6-anhydro-Galp (AnGalp) units. Interestingly, the more sulfated BoSG fraction showed slightly reduced in vitro anti-SARS-CoV-2 activities against both wild-type and delta variants, and significantly reduced anticoagulant activity. The BoSG fractions showed no cytotoxic effects. The reduction in both bioactivities is attributed to the presence of the AnGalp unit. Docking scores from computational simulations using BoSG disaccharide constructs on wild-type and delta S-proteins, and binding analysis through competitive SPR assays using blood (co)-factors (antithrombin, heparin cofactor II and thrombin) and four S-proteins (wild-type, delta, gamma, and omicron) strongly support the conclusion about the deleterious impact of the AnGalp unit.

Indexed as

COVID-19RhodophytaAnticoagulantsDisaccharidesGalactansHumansSARS-CoV-2SulfatesAnticoagulantsDisaccharidesGalactansSulfatesAnticoagulationAnti-SARS-CoV-2Botryocladia occidentalisStructural determinationSulfated galactan

Identifiers

PMID36963552
PMCPMC10175164
OpenAlexW4353055793

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.