HpaII-assisted and linear amplification-enhanced isothermal exponential amplification fluorescent strategy for rapid and sensitive detection of DNA methyltransferase activity.

Yaqian An, Zhiqi Yu, Di Liu, Lirong Han, Xian Zhang, Xuelian Xin, Cuiping Li

Abstract read

In one paragraph

Article in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

1.8field-weighted citation impact, top 15% of its field

1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yaqian AnKey Laboratory of Public Health Safety of Hebei Province, School of Public Health, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding, 071002, People's Republic of China.

Zhiqi YuKey Laboratory of Public Health Safety of Hebei Province, School of Public Health, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding, 071002, People's Republic of China.

Di LiuKey Laboratory of Public Health Safety of Hebei Province, School of Public Health, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding, 071002, People's Republic of China.

Lirong HanKey Laboratory of Public Health Safety of Hebei Province, School of Public Health, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding, 071002, People's Republic of China.

Xian ZhangKey Laboratory of Public Health Safety of Hebei Province, School of Public Health, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding, 071002, People's Republic of China.

Xuelian XinKey Laboratory of Public Health Safety of Hebei Province, School of Public Health, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding, 071002, People's Republic of China.

Cuiping LiKey Laboratory of Public Health Safety of Hebei Province, School of Public Health, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding, 071002, People's Republic of China. cuipingli@hbu.edu.cn.

Hebei University · CN

Funding

National Natural Science Foundation of China 21707027

6 · The paper itself

Abstract

The detection of methyltransferase (MTase) activity is of great significance in methylation-related disease diagnosis and drug screening. Herein, a HpaII-assisted and linear amplification-enhanced exponential amplification strategy is proposed for sensitive and label-free detection of M.SssI MTase activity. The P1 probe contains self-complementary sequence 5'-CTAGCCGGCTAG-3' at 3'-terminal. After denaturation and annealing, P1 probes hybridize with itself to generate P1 duplexes. M.SssI MTase induces methylation of cytosine at 5'-CG-3' in P1 duplexes, and thus, HpaII fails to cleave at 5'-CCGG-3' due to methylation sensitivity, leaving P1 duplex intact. Then, these intact P1 duplexes are extended along 3'-terminal through Vent (exo

Indexed as

Biosensing TechniquesDNADNA MethylationDNA Modification MethylasesDNA, Single-StrandedHumansDNADNA Modification MethylasesDNA, Single-StrandedFluorescent sensorHpaIIIsothermal exponential amplification reactionM.SssI DNA methyltransferase activity

Identifiers

PMID36961574

DOI10.1021/acs.analchem.6b00450

OpenAlexW4360817274

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.