Evidence map›Paper›PMID 36952186›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Assessment of the Peroxisomal Redox State in Living Cells Using NADPH- and NAD

Cláudio F Costa, Hongli Li, Mohamed A F Hussein, Yi Yang, Celien Lismont, Marc Fransen

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.5field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

Cláudio F CostaDepartment of Cellular and Molecular Medicine, Laboratory of Peroxisome Biology and Intracellular Communication, KU Leuven, Leuven, Belgium.
Hongli LiDepartment of Cellular and Molecular Medicine, Laboratory of Peroxisome Biology and Intracellular Communication, KU Leuven, Leuven, Belgium.
Mohamed A F HusseinDepartment of Cellular and Molecular Medicine, Laboratory of Peroxisome Biology and Intracellular Communication, KU Leuven, Leuven, Belgium.
Yi YangSynthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, Shanghai, China.
Celien LismontDepartment of Cellular and Molecular Medicine, Laboratory of Peroxisome Biology and Intracellular Communication, KU Leuven, Leuven, Belgium.
Marc FransenDepartment of Cellular and Molecular Medicine, Laboratory of Peroxisome Biology and Intracellular Communication, KU Leuven, Leuven, Belgium. marc.fransen@kuleuven.be.
KU Leuven · BEAssiut University · EGEast China University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The pyridine nucleotides NAD(H) and NADP(H) are key molecules in cellular metabolism, and measuring their levels and oxidation states with spatiotemporal precision is of great value in biomedical research. Traditional methods to assess the redox state of these metabolites are intrusive and prohibit live-cell quantifications. This obstacle was surpassed by the development of genetically encoded fluorescent biosensors enabling dynamic measurements with subcellular resolution in living cells. Here, we provide step-by-step protocols to monitor the intraperoxisomal NADPH levels and NAD

Indexed as

NADLuminescent ProteinsNADPOxidation-ReductionLuminescent ProteinsNADNADPFluorescent biosensorsiNAPLive-cell imagingNAD(H)NADP(H)PeroxisomesRedox stateSoNar

Identifiers

PMID36952186
OpenAlexW4360600971

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.