ArticleNature cardiovascular research2023
Surfaceome mapping of primary human heart cells with CellSurfer uncovers cardiomyocyte surface protein LSMEM2 and proteome dynamics in failing hearts.
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Article in Nature cardiovascular research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32citing papers in PubMed
5.5field-weighted citation impact, top 3% of its field
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Who cites it
32 citing papers in PubMed, 37 citations in OpenAlex.
- Heart-specific T cell responses: from antigens to disease mechanisms.Nature reviews. Cardiology · 2026Review
- Cell-Surface Signatures and Targets of Modulated Vascular Smooth Muscle Cells in Atherosclerosis: From State Identification to Precision Intervention.Journal of cardiovascular development and disease · 2026Review
- Micro- and Macro-Heterogeneity of N-Glycoproteome Remodeling in Endoplasmic Reticulum Stress.Molecular & cellular proteomics : MCP · 2026Article
- Tuning Secretomes for Regenerative Medicine.Biology · 2026Review
- Development of Human iPSC-based Microphysiogical Models of Transthyretin Amyloid Cardiomyopathy.Research square · 2026Article
- Consensus statement on mass spectrometry-based proteomic analysis of cardiac tissue.Nature cardiovascular research · 2026Article
- Astrocyte targeted SMN1 gene therapy and forskolin application improves astrocyte filopodia actin defects and motor neuron synaptic dysfunction in human SMA disease pathology.bioRxiv : the preprint server for biology · 2026Article
- Pan-cancer N-glycoproteomic atlas of patient-derived xenografts uncovers FAT2 as an actionable surface target.Cell reports. Medicine · 2026Article
- Pharmacological considerations for next-generation protein therapeutics in cardiovascular disease.The Journal of pharmacology and experimental therapeutics · 2025Review
- Profiling the Cellular Surfaceome by Furan-Based Protein Biotinylation.Journal of proteome research · 2025Article
- Quantitative proteomics of formalin-fixed, paraffin-embedded cardiac specimens uncovers protein signatures of specialized regions and patient groups.Nature cardiovascular research · 2025Article
- BubR1 Insufficiency Drives Transcriptomic Alterations and Pathology Associated With Cardiac Aging and Heart Failure.Aging cell · 2025Article
- LSMEM2, Localized at the Neuromuscular Junction, Modulates Mitochondrial Integration in Skeletal Muscles.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Engineered Proteins and Chemical Tools to Probe the Cell Surface Proteome.Chemical reviews · 2025Review
- Integrated Multiomics Reveals Alterations in Paucimannose and Complex Type N-Glycans in Cardiac Tissue of Patients with COVID-19.Molecular & cellular proteomics : MCP · 2025Article
- Article
- Spatiotemporally resolved mapping of extracellular proteomes via in vivo-compatible TyroID.Nature communications · 2025Article
- Mass Spectrometry-Based Spatial Multiomics Revealed Bioaccumulation Preference and Region-Specific Responses of PFOS in Mice Cardiac Tissue.Environmental science & technology · 2025Article
- Cell-surface Milieu Remodeling in Human Dendritic Cell Activation.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- An engineered human cardiac tissue model reveals contributions of systemic lupus erythematosus autoantibodies to myocardial injury.Nature cardiovascular research · 2024Article
Corrections and comments
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Authors and funding
18 authors at 7 institutions in 2 countries.
Linda Berg LueckeCardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA.
Matthew WaasCardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA.
Jack LittrellCardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA.
Melinda WojtkiewiczCardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA.
Chase CastroCardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA.
Maria BurkovetskayaCardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA.
Erin N SchuetteCardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA.
Amanda Rae BuchbergerDepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA.
Jared M ChurkoDepartment of Cellular and Molecular Medicine and Sarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA.
Upendra ChaliseCardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA.ORCID 0000-0002-7798-7697
Michelle WaknitzCardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA.
Shelby KonfrstCardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA.
Roald TeubenDepartment of Biomedical Engineering, Whiting School of Engineering, The Johns Hopkins University, Baltimore, MD, USA.
Justin Morrissette-McAlmonDepartment of Biomedical Engineering, Whiting School of Engineering, The Johns Hopkins University, Baltimore, MD, USA.
Claudius MahrDepartment of Mechanical Engineering, Division of Cardiology, University of Washington, Seattle, WA, USA.
Daniel R AndersonDivision of Cardiovascular Medicine, University of Nebraska Medical Center, Omaha, NE, USA.
Kenneth R BohelerDepartment of Biomedical Engineering, Whiting School of Engineering, The Johns Hopkins University, Baltimore, MD, USA.
Rebekah L GundryCardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA.ORCID 0000-0002-9263-833X
University of Nebraska Medical Center · USJohns Hopkins University · USMedical College of Wisconsin · USPrincess Margaret Cancer Centre · CAUniversity of Arizona · USUniversity of Washington · USUniversity of Wisconsin–Madison · US
Funding
Medical Scientist Training ProgramT32GM080202 · NIGMS · MEDICAL COLLEGE OF WISCONSIN · PI BARBIERI, JOSEPH T, SALZMAN, NITA H · 2010 to 2024
$5.7MHarnessing Glycoproteomics and Glycomics to Understand Cardiac Biology and DiseaseR35HL155460 · NHLBI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI Rebekah L. Gundry · 2021 to 2026
$4.6MJ. NRSA Training CoreTL1TR001437 · NCATS · MEDICAL COLLEGE OF WISCONSIN · PI THOMAS, DANNY GEORGE · 2015 to 2024
$3.7MEngineered Human Heart Slice for Testing Drug-Induced ArrhythmiaR01HL152249 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI BOHELER, KENNETH RICHARD, KWON, CHULAN · 2020 to 2022
$2.0MCell Surface Phenotyping Human Primary CellsR01HL134010 · NHLBI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GUNDRY, REBEKAH L. · 2016 to 2020
$1.9MCell Surface Proteins in Human CardiomyocytesR01HL126785 · NHLBI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GUNDRY, REBEKAH L. · 2016 to 2019
$1.5MInvestigating the Utility and Function of a Novel Cardiomyocyte Cell Surface ProteinF31HL140914 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI WAAS, MATTHEW R. · 2018 to 2019
$70kNCATS NIH HHS TL1 TR001437NHLBI NIH HHS F31 HL140914NHLBI NIH HHS R01 HL126785NHLBI NIH HHS R01 HL134010NHLBI NIH HHS R01 HL152249NHLBI NIH HHS R35 HL155460NIGMS NIH HHS T32 GM080202
Abstract
Cardiac cell surface proteins are drug targets and useful biomarkers for discriminating among cellular phenotypes and disease states. Here we developed an analytical platform, CellSurfer, that enables quantitative cell surface proteome (surfaceome) profiling of cells present in limited quantities, and we apply it to isolated primary human heart cells. We report experimental evidence of surface localization and extracellular domains for 1,144
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