Evidence map›Paper›PMID 36946051›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2023

[Type III secretory protein SINC of

X Zeng, L Chen, P Zhou, T Tang, X Chen, D Hu, C Wang, L Chen

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

X ZengDepartment of Public Health Laboratory Sciences, School of Public Health, University of South China, Hengyang 421001, China.
L ChenDepartment of Public Health Laboratory Sciences, School of Public Health, University of South China, Hengyang 421001, China.
P ZhouDepartment of Public Health Laboratory Sciences, School of Public Health, University of South China, Hengyang 421001, China.
T TangDepartment of Public Health Laboratory Sciences, School of Public Health, University of South China, Hengyang 421001, China.
X ChenDepartment of Public Health Laboratory Sciences, School of Public Health, University of South China, Hengyang 421001, China.
D HuDepartment of Public Health Laboratory Sciences, School of Public Health, University of South China, Hengyang 421001, China.
C WangInstitute of Pathogen Biology, Hengyang Medical School, University of South China, Hengyang 421001, China.
L ChenDepartment of Public Health Laboratory Sciences, School of Public Health, University of South China, Hengyang 421001, China.
University of South China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the effects of SINC, a secreted protein of

methodsRAW 264.7 cells treated with recombinant SINC were examined for changes in expression levels of LC3-II, Beclin-1, phosphorylated and total ERK1/2 using Western blotting. The expression level of LC3 in the treated cells was detected using immunofluorescence analysis, and the formation of autophagosomes and autolysosomes was observed with transmission electron microscopy (TEM). The effect of pretreatment with U0126 (a specific ERK inhibitor) on the expression levels of LC3-II and Beclin-1 in RAW 264.7 cells exposed to different concentrations of SINC was examined using Western blotting, and LC3 puncta in the cells was detected with immunofluorescence analysis.

resultsThe expression levels of LC3-II and Beclin-1 were the highest in RAW 264.7 cells treated with 2 μg/mL SINC for 12h. Immunofluorescence analysis showed exposure to SINC significantly increased the number of cells containing LC3 puncta, where the presence of autophagosomes and autolysosomes was detected. Exposure to 2 μg/mL SINC for 15 min resulted in the most significant increase of the ratios of p-ERK1/2/ERK1/2 in RAW 264.7 cells. Pretreatment of the cells with U0126 prior to SINC exposure significantly decreased the ratio of p-ERK1/2/ERK1/2, lowered the expression levels of LC3-II and Beclin-1, and decreased LC3 aggregation in the cells.

conclusionsSINC exposure can induce autophagy in RAW 264.7 cells by activating the MAPK/ERK signaling pathway.

Indexed as

Chlamydophila psittaciMAP Kinase Signaling SystemAutophagyBeclin-1ButadienesNitrilesSignal TransductionSilanesBeclin-1bis(tri-n-hexylsiloxy)(2,3-naphthalocyaninato)siliconButadienesNitrilesSilanesU 0126autophagyChlamydophila psittaciMAPK/ERK signaling PathwaySINC

Identifiers

PMID36946051
PMCPMC10034536
OpenAlexW4353015165

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.