ArticlePlant communications2023
Boosting wheat functional genomics via an indexed EMS mutant library of KN9204.
Article in Plant communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 51 citations in OpenAlex.
- Deletion of TaBSK3-2B attenuates BR signaling to regulate the Rht4 semi-dwarf phenotype and drought tolerance in wheat.The Plant journal : for cell and molecular biology · 2026Article
- Introgressed Variation in TaMYB7-A1 Drives Graded Dormancy and Climate-Adaptive Pre-Harvest Sprouting Resistance in Wheat.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- Fine mapping and candidate gene identification of the stripe rust resistance gene Yr83 using mutated wheat-rye translocation lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata.Plant communications · 2026Article
- Telomere-to-telomere genome assembly and comprehensive mutation library facilitate bitter gourd breeding and functional genomics research.Plant communications · 2026Article
- Image-based rachis phenotyping facilitates genetic dissection of spikelet distribution in wheat.Plant physiology · 2026Article
- Establishment of anFrontiers in plant science · 2026Article
- Integrated Multi-Omics Reveals the Molecular Basis Underlying Wheat Grain Development and IdentifiesPlants (Basel, Switzerland) · 2025Article
- Population-scale gene expression analysis reveals the contribution of expression diversity to the modern wheat improvement.Nature communications · 2025Article
- A Forward Genetics Strategy for High-Throughput Gene Identification via Precise Image-Based Phenotyping of an Indexed EMS Mutant Library.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Increased Apigenin in DNA-Edited Hexaploid Wheat Promoted Soil Bacterial Nitrogen Fixation and Improved Grain Yield Under Limiting Nitrogen Fertiliser.Plant biotechnology journal · 2025Article
- Integrative omics of the genetic basis for wheat WUE and drought resilience reveal the function of TaMYB7-A1.Nature communications · 2025Article
- The impact of asymmetrical gene expression on the development of spike morphology iniMetaOmics · 2025Article
- Spatiotemporal transcriptomics reveals key gene regulation for grain yield and quality in wheat.Genome biology · 2025Article
- Creating a gene-indexed EMS mutation library of Zheng58 for improving maize genetics research.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Development and application of aFrontiers in plant science · 2025Article
- Partial unidirectional translocation from 5AL to 7BS leads to dense spike in an EMS-induced wheat mutant.BMC genomics · 2024Article
- Distinct roles of H3K27me3 and H3K36me3 in vernalization response, maintenance, and resetting in winter wheat.Science China. Life sciences · 2024Article
- Deciphering the Transcriptional Regulatory Network Governing Starch and Storage Protein Biosynthesis in Wheat for Breeding Improvement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
Corrections and comments
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Authors and funding
24 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A better understanding of wheat functional genomics can improve targeted breeding for better agronomic traits and environmental adaptation. However, the lack of gene-indexed mutants and the low transformation efficiency of wheat limit in-depth gene functional studies and genetic manipulation for breeding. In this study, we created a library for KN9204, a popular wheat variety in northern China, with a reference genome, transcriptome, and epigenome of different tissues, using ethyl methyl sulfonate (EMS) mutagenesis. This library contains a vast developmental diversity of critical tissues and transition stages. Exome capture sequencing of 2090 mutant lines using KN9204 genome-designed probes revealed that 98.79% of coding genes had mutations, and each line had an average of 1383 EMS-type SNPs. We identified new allelic variations for crucial agronomic trait-related genes such as Rht-D1, Q, TaTB1, and WFZP. We tested 100 lines with severe mutations in 80 NAC transcription factors (TFs) under drought and salinity stress and identified 13 lines with altered sensitivity. Further analysis of three lines using transcriptome and chromatin accessibility data revealed hundreds of direct NAC targets with altered transcription patterns under salt or drought stress, including SNAC1, DREB2B, CML16, and ZFP182, factors known to respond to abiotic stress. Thus, we have generated and indexed a KN9204 EMS mutant library that can facilitate functional genomics research and offer resources for genetic manipulation of wheat.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.