Evidence map›Paper›PMID 36945534›Full record

ArticlebioRxiv : the preprint server for biology2023

Label-free proteomic comparison reveals ciliary and non-ciliary phenotypes of IFT-A mutants.

Janelle C Leggere, Jaime V K Hibbard, Ophelia Papoulas, Chanjae Lee, Chad G Pearson, Edward M Marcotte, John B Wallingford

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Janelle C Leggere
Jaime V K HibbardORCID 0000-0003-4993-5213
Ophelia PapoulasORCID 0000-0002-6370-0616
Chanjae Lee
Chad G PearsonORCID 0000-0003-1915-6593
Edward M MarcotteORCID 0000-0001-8808-180X
John B WallingfordORCID 0000-0001-8701-4293
The University of Texas at Austin · USUniversity of Colorado Anschutz Medical Campus · US

Funding

Mapping the CPLANE interactome, an extensive protein interaction network underlying human ciliopathiesR01HD085901 · NICHD · UNIVERSITY OF TEXAS AT AUSTIN · PI EDWARD M MARCOTTE, John B Wallingford · 2016 to 2026
$6.2M
Proteomics and model organism humanization to decode human geneticsR35GM122480 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI EDWARD M MARCOTTE · 2017 to 2026
$5.4M
Centriole assembly and function for centrosome and cilia biologyR35GM140813 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI CHAD G PEARSON · 2021 to 2026
$3.5M
NICHD NIH HHS R01 HD085901NIGMS NIH HHS R35 GM122480NIGMS NIH HHS R35 GM140813
6 · The paper itself

Abstract

DIFFRAC is a powerful method for systematically comparing proteome content and organization between samples in a high-throughput manner. By subjecting control and experimental protein extracts to native chromatography and quantifying the contents of each fraction using mass spectrometry, it enables the quantitative detection of alterations to protein complexes and abundances. Here, we applied DIFFRAC to investigate the consequences of genetic loss of Ift122, a subunit of the intraflagellar transport-A (IFT-A) protein complex that plays a vital role in the formation and function of cilia and flagella, on the proteome of

Identifiers

PMID36945534
PMCPMC10028850
OpenAlexW4323657519

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.