Evidence map›Paper›PMID 36945456›Full record

ArticlebioRxiv : the preprint server for biology2024

Conserved cysteine residues in Kaposi's sarcoma herpesvirus ORF34 are necessary for viral production and viral pre-initiation complex formation.

Tadashi Watanabe, Aidan McGraw, Kedhar Narayan, Hasset Tibebe, Kazushi Kuriyama, Mayu Nishimura, Taisuke Izumi, Masahiro Fujimuro, Shinji Ohno

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Tadashi WatanabeDepartment of Virology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Nakagami, Okinawa, 903-0215, Japan.
Aidan McGrawDepartment of Biology, College of Arts & Sciences, American University, Washington, D.C., 20016, U.S.A.
Kedhar NarayanDepartment of Biology, College of Arts & Sciences, American University, Washington, D.C., 20016, U.S.A.
Hasset TibebeDepartment of Biology, College of Arts & Sciences, American University, Washington, D.C., 20016, U.S.A.
Kazushi KuriyamaDepartment of Virology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Nakagami, Okinawa, 903-0215, Japan.
Mayu NishimuraDepartment of Cell Biology, Kyoto Pharmaceutical University, 1 Misasagi-Shichono, Yamashina, Kyoto 607-8412, Japan.
Taisuke IzumiDepartment of Biology, College of Arts & Sciences, American University, Washington, D.C., 20016, U.S.A.
Masahiro FujimuroDepartment of Cell Biology, Kyoto Pharmaceutical University, 1 Misasagi-Shichono, Yamashina, Kyoto 607-8412, Japan.ORCID 0000-0002-5165-0789
Shinji OhnoDepartment of Virology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Nakagami, Okinawa, 903-0215, Japan.
American University · USUniversity of the Ryukyus · JPKyoto Pharmaceutical University · JP

Funding

Role of canonical and non-canonical beta-catenin signaling in HIV-1 latencyR15AI172610 · NIAID · SAINT JOSEPH'S UNIVERSITY · PI Taisuke Izumi · 2022 to 2026
$885k
NIAID NIH HHS R15 AI172610
6 · The paper itself

Abstract

Kaposi's sarcoma herpesvirus (KSHV) ORF34 plays a significant role as a component of the viral pre-initiation complex (vPIC), which is indispensable for late gene expression across beta and gamma herpesviruses. Although the key role of ORF34 within the vPIC and its function as a hub protein have been recognized, further clarification regarding its specific contribution to vPIC functionality and interactions with other components is required. This study employed a deep-learning algorithm-assisted structural model of ORF34, revealing highly conserved amino acid residues across human beta- and gamma-herpesviruses localized in structured domains. Thus, we engineered ORF34 alanine-scanning mutants by substituting conserved residues with alanine. These mutants were evaluated for their ability to interact with other vPIC factors and restore viral production in cells harboring the ORF34-deficient KSHV-BAC. Our experimental results highlight the crucial role of the 4 cysteine residues conserved in ORF34: a tetrahedral arrangement consisting of a pair of C-X

Identifiers

PMID36945456
PMCPMC10028899
OpenAlexW4323859698

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.