Evidence map›Paper›PMID 36945027›Full record

ArticlePlant methods2023

Transmembrane potential, an indicator in situ reporting cellular senescence and stress response in plant tissues.

Hai Liu, Yufei Li, Ting Peng, Shaowu Xue

Open access · goldFull text read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.6field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Hai Liu *College of Life Science and Technology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.
Yufei Li *College of Life Science and Technology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.
Ting PengCollege of Agriculture, Guizhou University, Guiyang, 550025, China.
Shaowu XueCollege of Life Science and Technology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China. xues@mail.hzau.edu.cn.
Huazhong Agricultural University · CNGuizhou University · CN

Funding

Fundamental Research Funds for the Central Universities 2662020SKY008Guizhou Provincial Science and Technology Support Project 2018-2302National Natural Science Foundation of China 32070214Young Science and Technology Talents Project of Department of Education of Guizhou Province 2017-112
6 · The paper itself

Abstract

backgroundPlant cells usually sustain a stable membrane potential due to influx and/or efflux of charged ions across plasma membrane. With the growth and development of plants, different tissues and cells undergo systemic or local programmed decline. Whether the membrane potential of plasma membrane could report senescence signal of plant tissues and cells is unclear.

resultsWe applied a maneuverable transmembrane potential (TMP) detection method with patch-clamp setup to examine the senescence signal of leaf tissue cells in situ over the whole life cycle in Arabidopsis thaliana. The data showed that the TMPs of plant tissues and cells were varied at different growth stages, and the change of TMP was higher at the vegetative growth stage than at the reproductive stage of plant growth. The distinct change of TMP was detectable between the normal and the senescent tissues and cells in several plant species. Moreover, diverse abiotic stimuli, such as heat stress, hyperpolarized the TMP in a short time, followed by depolarized membrane potential with the senescence occurring. We further examined the TMP of plant chloroplasts, which also indicates the senescence signal in organelles.

conclusionsThis convenient TMP detection method can report the senescence signal of plant tissues and cells, and can also indicate the potential of plant tolerance to environmental stress.

Indexed as

Abiotic stressDepolarizationHyperpolarizationOrganellesSenescence signalTransmembrane potential

Identifiers

PMID36945027
PMCPMC10029184
OpenAlexW4353018868

What OpenQuestion holds

Textfull text, public
LicenceCC BY
measurements read71
table measurements read3
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.