Evidence map›Paper›PMID 36944890›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Live Whole-Plant Detection of Rapidly Accumulating Reactive Oxygen Species Following Applied Stress in Arabidopsis thaliana.

Ronald J Myers, Sara I Zandalinas, Ron Mittler

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.5field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Ronald J MyersDivision of Plant Sciences and Technology, College of Agriculture Food and Natural Resources and Interdisciplinary Plant Group, University of Missouri, Columbia, MO, USA.
Sara I ZandalinasDepartment of Biology, Biochemistry and Environmental Sciences, University Jaume I, Castelló de la Plana, Spain.
Ron MittlerDivision of Plant Sciences and Technology, College of Agriculture Food and Natural Resources and Interdisciplinary Plant Group, University of Missouri, Columbia, MO, USA. mittlerr@missouri.edu.ORCID 0000-0003-3192-7450
Plant (United States) · USUniversitat Jaume I · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reactive Oxygen Species (ROS) waves serve as key systemic signals within plants. Following the initial sensation of a stress, auto-propagation of ROS (the ROS wave) begins and rapidly spreads to distant, systemic tissues of the plant and invokes important physiological responses. Highly sensitive methods capable of imaging this systemic signal at the whole-plant level have long been desired for the study of ROS signaling. Here, we describe a straightforward and highly sensitive method for the detection and quantification of ROS in planta at the whole-plant level in Arabidopsis thaliana with the In Vivo Imaging System (IVIS) Lumina S5 imaging platform and the fluorescent probe 2',7'-dichlorofluorescin diacetate (H

Indexed as

ArabidopsisArabidopsis ProteinsReactive Oxygen SpeciesStress, PhysiologicalArabidopsis ProteinsReactive Oxygen SpeciesArabidopsisData acquisitionH2DCFDAImagingIn vivoROSStressSystemic signalingWhole-plant

Identifiers

PMID36944890
OpenAlexW4352977332

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.