ArticleCommunications biology2023
Gradient to sectioning CUBE workflow for the generation and imaging of organoids with localized differentiation.
Article in Communications biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.
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Who cites it
7 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Mapping the Cerebral Organoid Landscape: A Systematic Review of Preclinical 3D Models in Neuroscience.Advanced healthcare materials · 2026Pooled it
- Effects of size scaling on cellular dynamics and tissue patterning in tooth root organoids.Frontiers in bioengineering and biotechnology · 2026Article
- Specification of human brain regions with orthogonal gradients of WNT and SHH in organoids reveals patterning variations across cell lines.Cell stem cell · 2025Article
- Modeling of skeletal development and diseases using human pluripotent stem cells.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2024Review
- Modular tissue-in-a-CUBE platform to model blood-brain barrier (BBB) and brain interaction.Communications biology · 2024Article
- How might we build limbsFrontiers in cell and developmental biology · 2023Review
- 3D multicellular systems in disease modelling: From organoids to organ-on-chip.Frontiers in cell and developmental biology · 2023Review
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Authors and funding
2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Advancements in organoid culture have led to various in vitro mini-organs that mimic native tissues in many ways. Yet, the bottleneck remains to generate complex organoids with body axis patterning, as well as keeping the orientation of organoids during post-experiment analysis processes. Here, we present a workflow for culturing organoids with morphogen gradient using a CUBE culture device, followed by sectioning samples with the CUBE to retain information on gradient direction. We show that hiPSC spheroids cultured with two separated differentiation media on opposing ends of the CUBE resulted in localized expressions of the respective differentiation markers, in contrast to homogeneous distribution of markers in controls. We also describe the processes for cryo and paraffin sectioning of spheroids in CUBE to retain gradient orientation information. This workflow from gradient culture to sectioning with CUBE can provide researchers with a convenient tool to generate increasingly complex organoids and study their developmental processes in vitro.
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