Evidence map›Paper›PMID 36944703›Full record

ArticlePlanta2023

Purification, characterization and inactivation kinetics of polyphenol oxidase extracted from Cistanche deserticola.

Jin Huang, Xiaoguang Gao, Lingling Su, Xueqiang Liu, Limin Guo, Zhentao Zhang, Dandan Zhao, Jianxiong Hao

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Recent Advances Regarding Polyphenol Oxidase inFoods (Basel, Switzerland) · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Jin HuangCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang, 050000, China.
Xiaoguang GaoCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang, 050000, China.
Lingling SuCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang, 050000, China.
Xueqiang LiuCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang, 050000, China.
Limin GuoInstitute of Agro-Production Storage and Processing, Xinjiang Academy of Agricultural Sciences, Ürümqi, 830091, China.
Zhentao ZhangTechnical Institute of Physics and Chemistry CAS, Beijing, 100190, China.
Dandan ZhaoCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang, 050000, China. zdd2016@hebust.edu.cn.ORCID http://orcid.org/0000-0003-2315-6190
Jianxiong HaoCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang, 050000, China. hjxcau@163.com.
Hebei University of Science and Technology · CNTechnical Institute of Physics and Chemistry · CNXinjiang Academy of Agricultural Sciences · CN

Funding

National Nature Science Foundation 32101878Regional Collaborative Innovation Project of The Autonomous Region 2020E01048Science and Technology Project of Hebei Education Department QN2022162
6 · The paper itself

Abstract

MAIN

conclusionPPO was purified from Cistanche deserticola, and its enzymatic characteristics were clarified. It was found that microwave treatment was an efficient way to inactivate PPO. Polyphenol oxidase (PPO) from Cistanche deserticola was obtained and purified through an acetone precipitation and anion exchange column, the enzymatic characteristics and inactivation kinetics of PPO were studied. The specific activity of PPO was 73135.15 ± 6625.7 U/mg after purification, the purification multiple was 48.91 ± 4.43 times, and the recovery was 30.96 ± 0.27%. The molecular weight of the PPO component is about 66 kDa by SDS-PAGE analysis. The optimum substrate of PPO was catechol (V

Indexed as

CistancheCatechol OxidaseHydrogen-Ion ConcentrationKineticsMolecular WeightTemperatureCatechol OxidaseCistanche deserticolaEnzymatic propertiesInactivation of enzymePolyphenol oxidasePurification

Identifiers

PMID36944703
OpenAlexW4353016386

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.