Evidence map›Paper›PMID 36944665›Full record

ArticleScientific reports2023

Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare.

Mariana Diel de Amorim, Lynn Dong, Michael Byron, Robert A Foster, Claudia Klein, Monique Saleh, Tarek Saleh, Claire Card

Open access · goldFull text read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Mariana Diel de AmorimDepartment of Clinical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY, 14853, USA. md649@cornell.edu.
Lynn DongImmunopathology Research and Development Laboratory, Department of Biomedical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY, 14853, USA.
Michael ByronDepartment of Clinical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY, 14853, USA.
Robert A FosterDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON, N1G 2W1, Canada.
Claudia KleinDepartment of Veterinary Clinical and Diagnostic Science, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.
Monique SalehDepartment of Biomedical Sciences, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON, N1G 2W1, Canada.
Tarek SalehDepartment of Biomedical Sciences, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON, N1G 2W1, Canada.
Claire CardDepartment of Large Animal Clinical Sciences, Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada. claire.card@usask.ca.
Cornell University · USUniversity of Guelph · CAUniversity of Calgary · CAUniversity of Saskatchewan · CA

Funding

Acquisition of a Hybrid Quadrupole Time of Flight LC-MS/MS System for the CornellS10OD017992 · OD · CORNELL UNIVERSITY · PI ZHANG, SHENG · 2014 to 2014
$600k
NIH HHS S10 OD017992
6 · The paper itself

Abstract

Oxytocin is a hormone with functions in: reproduction, maternal bonding, milk ejection, and feeding/social behavior, and is reported to be present in a variety of tissues. Our goal is to characterize oxytocin and leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase), a key regulator of oxytocin in mares. We measured serum and tissue LNPEP by ELISA from ovulation (D0) until D21-22 in non-pregnant (n = 5) and pregnant mares (n = 6); and in periparturient and postpartum mares (n = 18). Placenta (n = 7) and homogenized tissue of diestrus mares (n = 6) were evaluated using protein determinations and LNPEP ELISAs. Identification of LNPEP and OXT protein in tissues was also performed via western blot, immunohistochemistry and liquid chromatography-mass spectrometry (LC-MS/MS). Furthermore, in situ hybridization was performed for LNPEP and OXT on endometrium, myometrium, pituitary and corpus luteum (CL). Serum LNPEP concentration were similar. Placental LNPEP U/mg protein was highest in the body and pregnant horn. The highest to lowest LNPEP U/mg protein by tissue were: myometrium > follicle wall > endometrium > kidney > CL > liver. Oxytocin was identified in the equine pituitary, CL and placenta and is likely to act in autocrine or paracrine manner, while LNPEP may act systemically and locally to regulate the availability of OXT.

Indexed as

Cystinyl AminopeptidaseOxytocinAnimalsChromatography, LiquidFemaleHorsesPlacentaPregnancyTandem Mass SpectrometryCystinyl AminopeptidaseOxytocin

Identifiers

PMID36944665
PMCPMC10030782
OpenAlexW4328102516

What OpenQuestion holds

Textfull text, public
LicenceCC BY
measurements read58
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.