ArticlePloS one2023
Comparative metabolomics analysis reveals alkaloid repertoires in young and mature Mitragyna speciosa (Korth.) Havil. Leaves.
Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- Natural Deep Eutectic Solvent Extraction Optimization of Alkaloids from Mitragyna speciosa: A Methodological Evaluation.Planta medica · 2026Article
- Solvent-Dependent Green Synthesis of ZnO Nanopowders UsingGels (Basel, Switzerland) · 2026Article
- Effects of Botanical Variation, Plant Part, and Extraction Technique on Mitragynine and 7-Hydroxymitragynine in Kratom (Molecules (Basel, Switzerland) · 2026Article
- Kratom (Molecules (Basel, Switzerland) · 2026Article
- Environmental Influence on the Untargeted Foliar Metabolome of Naturally GrowingPlant-environment interactions (Hoboken, N.J.) · 2026Article
- Kratom medium-chain dehydrogenase/reductase enzymes exhibit broad substrate and cofactor promiscuity.Journal of industrial microbiology & biotechnology · 2026Article
- Integrating spatial mapping and metabolomics: A novel platform for bioactive compound discovery and saline land reclamation.Computational and structural biotechnology journal · 2025Article
- Higher accumulation of mitragynine in Mitragyna speciosa (kratom) leaves affected by insect attack.PloS one · 2025Article
- Leaf color as an indicator of the accumulation pattern of pharmaco-nutritional compounds inFrontiers in plant science · 2025Article
- A comparative study of the quality differences and seasonal dynamics of flavonoids between the aerial parts and roots ofFrontiers in plant science · 2024Article
- Changes in the Metabolome and Nutritional Quality of Pulp from Three Types of Korla Fragrant Pears with Different Appearances as Revealed by Widely Targeted Metabolomics.Plants (Basel, Switzerland) · 2023Article
- Examining the Psychoactive Differences between Kratom Strains.International journal of environmental research and public health · 2023Article
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10 authors.
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Abstract
The fresh leaves of Mitragyna speciosa (Korth.) Havil. have been traditionally consumed for centuries in Southeast Asia for its healing properties. Although the alkaloids of M. speciosa have been studied since the 1920s, comparative and systematic studies of metabolite composition based on different leaf maturity levels are still lacking. This study assessed the secondary metabolite composition in two different leaf stages (young and mature) of M. speciosa, using an untargeted liquid chromatography-electrospray ionisation-time-of-flight-mass spectrometry (LC-ESI-TOF-MS) metabolite profiling. The results revealed 86 putatively annotated metabolite features (RT:m/z value) comprising 63 alkaloids, 10 flavonoids, 6 terpenoids, 3 phenylpropanoids, and 1 of each carboxylic acid, glucoside, phenol, and phenolic aldehyde. The alkaloid features were further categorised into 14 subclasses, i.e., the most abundant class of secondary metabolites identified. As per previous reports, indole alkaloids are the most abundant alkaloid subclass in M. speciosa. The result of multivariate analysis (MVA) using principal component analysis (PCA) showed a clear separation of 92.8% between the young and mature leaf samples, indicating a high variance in metabolite levels between them. Akuammidine, alstonine, tryptamine, and yohimbine were tentatively identified among the many new alkaloids reported in this study, depicting the diverse biological activities of M. speciosa. Besides delving into the knowledge of metabolite distribution in different leaf stages, these findings have extended the current alkaloid repository of M. speciosa for a better understanding of its pharmaceutical potential.
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