Evidence map›Paper›PMID 36943649›Full record

ArticlePhotochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology2023

Cloning and structural basis of fluorescent protein color variants from identical species of sea anemone, Diadumene lineata.

Yuki Horiuchi, Koki Makabe, Danai Laskaratou, Kuniyuki Hatori, Michel Sliwa, Hideaki Mizuno, Jun-Ichi Hotta

Abstract read
PubMed Publisher
In one paragraph

Article in Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yuki HoriuchiGraduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata, 992-8510, Japan.
Koki MakabeGraduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata, 992-8510, Japan.
Danai LaskaratouBiomolecular Network Dynamics, Biochemistry, Molecular and Structural Biology Section, KU Leuven, Celestijnenlaan 200g, Post Box 2403, 3001, Leuven, Belgium.
Kuniyuki HatoriGraduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata, 992-8510, Japan.
Michel SliwaUniv. Lille, CNRS, UMR 8516, LASIRE, LAboratoire de Spectroscopie pour les Interactions, la Réactivité et l'Environnement, 59000, Lille, France.
Hideaki MizunoBiomolecular Network Dynamics, Biochemistry, Molecular and Structural Biology Section, KU Leuven, Celestijnenlaan 200g, Post Box 2403, 3001, Leuven, Belgium.
Jun-Ichi HottaGraduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata, 992-8510, Japan. hotta@yz.yamagata-u.ac.jp.ORCID http://orcid.org/0000-0001-5729-7990

Funding

Institut Chevreul FR 2638KU Leuven C14/16/ 053Precursory Research for Embryonic Science and Technology JPMJPR10QA
6 · The paper itself

Abstract

Diadumene lineata is a colorful sea anemone with orange stripe tissue of the body column and plain tentacles with red lines. We subjected Diadumene lineata to expression cloning and obtained genes encoding orange (OFP: DiLiFP561) and red fluorescent proteins (RFPs: DiLiFP570 and DiLiFP571). These proteins formed obligatory tetramers. All three proteins showed bright fluorescence with the brightness of 58.3 mM

Indexed as

Sea AnemonesAnimalsCloning, MolecularColoring AgentsLuminescent ProteinsMutationProtein EngineeringColoring AgentsLuminescent ProteinsCrystal structureDiadumene lineataExpression cloningFluorescent proteinProtein expression

Identifiers

PMID36943649

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.