Evidence map›Paper›PMID 36943640›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2023

Molecular characterization of persistent subclinical mastitis-causing Staphylococcus aureus from dairy farms.

Juliano Leonel Gonçalves, Sarah H I Lee, Carlos H Camargo, Rosemeire Cobo Zanella, Nathália C C Silva, Vera L M Rall, Roger I Cue, Marcos V Dos Santos

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In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Juliano Leonel GonçalvesDepartment of Animal Nutrition and Production, School of Veterinary Medicine and Animal Sciences, University of Sao Paulo, Pirassununga, São Paulo, 13635-900, Brazil. julianolg@usp.br.ORCID http://orcid.org/0000-0003-2138-4982
Sarah H I LeeDepartment of Food Engineering, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, 13635-900, Brazil.
Carlos H CamargoCentro de Bacteriologia, Instituto Adolfo Lutz (IAL), São Paulo, SP, Brazil.
Rosemeire Cobo ZanellaCentro de Bacteriologia, Instituto Adolfo Lutz (IAL), São Paulo, SP, Brazil.
Nathália C C SilvaDepartment of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas, Campinas, São Paulo, Brazil.
Vera L M RallDepartment of Chemical and Biological Sciences, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
Roger I CueDepartment of Animal Science, Macdonald Campus, McGill University, Quebec, H9X 3V9, Canada.
Marcos V Dos SantosDepartment of Animal Nutrition and Production, School of Veterinary Medicine and Animal Sciences, University of Sao Paulo, Pirassununga, São Paulo, 13635-900, Brazil.

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo Proc. BEPE 2015/04570-1)Fundação de Amparo à Pesquisa do Estado de São Paulo project funding (Proc. 2014/17411-6).Fundação de Amparo à Pesquisa do Estado de São Paulo The authors acknowledge FAPESP for the scholarship (Proc. 2013/23613-8
6 · The paper itself

Abstract

The study aimed to evaluate the genetic diversity of Staphylococcus aureus causing subclinical mastitis (SM) isolated from dairy cows and to assess the effect of the infection status (transient vs. persistent) on the milk and component yield. A total of six dairy farms in São Paulo state were used for the selection of cows with SM caused by S. aureus. S. aureus strains (n = 56) obtained from three biweekly aseptic mammary quarter milk samplings (n = 1140 from 95 cows) were subjected to MALDI-TOF MS analysis for species confirmation and further PFGE analysis. Intramammary infections (IMI) caused by S. aureus were categorized as transient (T: when only one out of 3 milk samplings had positive isolation of any pulsotype) or persistent (P: when two (P2) or three (P3) milk samplings had positive isolation of identical pulsotype over the consecutive episodes of SM. The SmaI macrorestriction fragment profiles of 56 S. aureus isolates showed a dominant S. aureus clonal pattern (PFGE type A; n = 50; 89.3%) within and among the herds. The SM-causing S. aureus represented a reduction of quarter milk yield of 26.2% in transient and 54.8% in persistent cases as well as a reduction of total solid yield of 38.1% and 49.4%, respectively, when compared with the healthy control quarters. Overall, the greater chance of S. aureus to be persistent is when a dominant clonal pattern is present in the herd which consequently may be associated with the cause of accentuated milk loss.

Indexed as

Mastitis, BovineStaphylococcal InfectionsAnimalsBrazilCattleFarmsFemaleHumansMilkStaphylococcus aureusChronic infectionGenetic diversityMilk reductionPFGEStaphylococci

Identifiers

PMID36943640
PMCPMC10235307

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.