Evidence map›Paper›PMID 36943541›Full record

ReviewNano convergence2023

Spectroscopic single-molecule localization microscopy: applications and prospective.

Benjamin Brenner, Cheng Sun, Françisco M Raymo, Hao F Zhang

Full text readReview
In one paragraph

Review in Nano convergence, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Chromatix: a differentiable, GPU-accelerated wave-optics library.bioRxiv : the preprint server for biology · 2026
    Article
  7. Review
  8. Advanced optical microscopy methods forBiophysical reviews · 2025
    Review
  9. Article
  10. Review
  11. Article
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Benjamin BrennerDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Cheng SunDepartment of Mechanical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Françisco M RaymoDepartment of Chemistry, University of Miami, Coral Gables, FL, 33146, USA.
Hao F ZhangDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA. hfzhang@northwestern.edu.ORCID http://orcid.org/0000-0001-5089-1196

Funding

Technology Development UnitU54CA268084 · NCI · NORTHWESTERN UNIVERSITY · PI Daniela E Matei · 2022 to 2026
$10.0M
Chemistry of Life Processes Predoctoral Training ProgramT32GM105538 · NIGMS · NORTHWESTERN UNIVERSITY · PI KELLEHER, NEIL L · 2013 to 2022
$2.7M
Photoactivatable Fluorophores for High-Throughput Multiplexed Tracking of Single-Molecules in Live CellsR01GM143397 · NIGMS · UNIVERSITY OF MIAMI CORAL GABLES · PI RAYMO, FRANCISCO M · 2022 to 2025
$1.4M
Intrinsic-contrast super-resolution imaging of DNA at 2-nm resolutionR01GM139151 · NIGMS · NORTHWESTERN UNIVERSITY · PI ZHANG, HAO F · 2020 to 2023
$1.3M
Development of spectroscopic Single-Molecule Localization MicroscopyR01GM140478 · NIGMS · NORTHWESTERN UNIVERSITY · PI SUN, CHENG, ZHANG, HAO F · 2021 to 2024
$1.2M
Bright and switchable fluorophores for highly multiplexed super-resolution microscopy towards molecular interaction imagingR21GM141675 · NIGMS · NORTHWESTERN UNIVERSITY · PI ZHANG, YANG · 2021 to 2022
$400k
NCI NIH HHS U54 CA268084NIGMS NIH HHS R01 GM139151NIGMS NIH HHS R01GM139151NIGMS NIH HHS R01 GM140478NIGMS NIH HHS R01GM140478NIGMS NIH HHS R01 GM143397NIGMS NIH HHS R01GM143397NIGMS NIH HHS R21 GM141675NIGMS NIH HHS R21GM141675NIGMS NIH HHS T32 GM105538
6 · The paper itself

Abstract

Single-molecule localization microscopy (SMLM) breaks the optical diffraction limit by numerically localizing sparse fluorescence emitters to achieve super-resolution imaging. Spectroscopic SMLM or sSMLM further allows simultaneous spectroscopy and super-resolution imaging of fluorescence molecules. Hence, sSMLM can extract spectral features with single-molecule sensitivity, higher precision, and higher multiplexity than traditional multicolor microscopy modalities. These new capabilities enabled advanced multiplexed and functional cellular imaging applications. While sSMLM suffers from reduced spatial precision compared to conventional SMLM due to splitting photons to form spatial and spectral images, several methods have been reported to mitigate these weaknesses through innovative optical design and image processing techniques. This review summarizes the recent progress in sSMLM, its applications, and our perspective on future work.

Indexed as

Polarity sensingSingle-molecule localization microscopySingle particle trackingSpectroscopySuper-resolution microscopy

Identifiers

PMID36943541
PMCPMC10030755

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.