Evidence map›Paper›PMID 36939874›Full record

ArticleEuropean biophysics journal : EBJ2023

Investigation of dynamic solution interactions between NET-1 and UNC-5B by multi-wavelength analytical ultracentrifugation.

Haben Gabir, Monika Gupta, Markus Meier, Fabian Heide, Manuel Koch, Joerg Stetefeld, Borries Demeler

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in European biophysics journal : EBJ, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Haben GabirDepartment of Chemistry, University of Manitoba, Winnipeg, MB, Canada.
Monika GuptaGenvira Biosciences, Ottawa, ON, Canada.
Markus MeierDepartment of Chemistry, University of Manitoba, Winnipeg, MB, Canada.
Fabian HeideDepartment of Chemistry, University of Manitoba, Winnipeg, MB, Canada.
Manuel KochMedical Faculty, Institute for Dental Research and Oral Musculoskeletal Biology, University of Cologne, Cologne, Germany.
Joerg StetefeldDepartment of Chemistry, University of Manitoba, Winnipeg, MB, Canada.
Borries DemelerDepartment of Chemistry and Biochemistry, University of Lethbridge, Lethbridge, AB, Canada. demeler@gmail.com.ORCID http://orcid.org/0000-0002-2414-9518

Funding

Development of an UltraScan Meta-Scheduler for HPC Job SubmissionR01GM120600 · NIGMS · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI Emre H. Brookes, BORRIES DEMELER · 2016 to 2026
$3.6M
CIHR 201610PJT-152935NIGMS NIH HHS R01 GM120600NIH HHS 1R01GM120600
6 · The paper itself

Abstract

NET-1 is a key chemotropic ligand that signals commissural axon migration and change in direction. NET-1 and its receptor UNC-5B switch axon growth cones from attraction to repulsion. The biophysical properties of the NET-1 + UNC-5B complex have been poorly characterized. Using multi-wavelength-AUC by adding a fluorophore to UNC-5B, we were able to separate the UNC-5B sedimentation from NET-1. Using both multi-wavelength- and single-wavelength AUC, we investigated NET-1 and UNC-5B hydrodynamic parameters and complex formation. The sedimentation velocity experiments show that NET-1 exists in a monomer-dimer equilibrium. A close study of the association shows that NET-1 forms a pH-sensitive dimer that interacts in an anti-parallel orientation. UNC-5B can form equimolar NET-1 + UNC-5B heterocomplexes with both monomeric and dimeric NET-1.

Indexed as

Netrin-1Netrin ReceptorsProtein Interaction Domains and MotifsAnimalsHumansUltracentrifugationNetrin-1Netrin ReceptorsAxon guidanceHetero-interaction studies between proteinsHydrodynamic bead modelingMulti-wavelength analytical ultracentrifugationNetrin-1UltraScanUltraScan-SOMOUncoordinated-5

Identifiers

PMID36939874
PMCPMC10509325

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.