Evidence map›Paper›PMID 36939332›Full record

ArticleInfection and immunity2023

Recombinant Fusion Protein Vaccine Containing Clostridioides difficile FliC and FliD Protects Mice against C. difficile Infection.

Shaohui Wang, Xianghong Ju, Joshua Heuler, Keshan Zhang, Zhibian Duan, Hiran Malinda Lamabadu Warnakulasuriya Patabendige, Song Zhao, Xingmin Sun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infection and immunity, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. AgainstToxins · 2025
    Review
  3. Journal of bacteriology · 2025
    Review
  4. Article
  5. Article
  6. The state of play of rodent models for the study ofJournal of medical microbiology · 2024
    Review
  7. Host Immune Responses toTropical medicine and infectious disease · 2023
    Review
  8. Article
  9. Non-Toxin-BasedPathogens (Basel, Switzerland) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shaohui Wang *Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, Florida, USA.
Xianghong Ju *Department of Infectious Diseases and Global Health, Tufts University Cummings School of Veterinary Medicine, North Grafton, Massachusetts, USA.
Joshua HeulerDepartment of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, Florida, USA.
Keshan ZhangDepartment of Infectious Diseases and Global Health, Tufts University Cummings School of Veterinary Medicine, North Grafton, Massachusetts, USA.ORCID 0000-0002-9258-0377
Zhibian DuanDepartment of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, Florida, USA.
Hiran Malinda Lamabadu Warnakulasuriya PatabendigeDepartment of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, Florida, USA.
Song ZhaoDepartment of Infectious Diseases and Global Health, Tufts University Cummings School of Veterinary Medicine, North Grafton, Massachusetts, USA.
Xingmin SunDepartment of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, Florida, USA.ORCID 0000-0002-8900-9161

Funding

Multivalent vaccines against Clostridium difficile infectionR01AI132711 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI Xingmin Sun, Shifeng Wang · 2017 to 2026
$3.3M
Novel polymer biomaterials combating C. difficile infectionR01AI149852 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI CAI, JIANFENG, SUN, XINGMIN · 2019 to 2023
$1.9M
Signaling pathway of TNF-alpha production and Clostridium difficile infection (CDK01DK092352 · NIDDK · UNIVERSITY OF SOUTH FLORIDA · PI SUN, XINGMIN · 2012 to 2016
$470k
Multivalent Bacillus mucosal vaccines against Clostridium difficile infectionR21AI113470 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI SUN, XINGMIN · 2015 to 2016
$438k
The role of tumor progression locus 2 (TPL2) in the pathogenesis of Clostridium difficile infection (CDI)R03DK112004 · NIDDK · UNIVERSITY OF SOUTH FLORIDA · PI SUN, XINGMIN · 2017 to 2018
$154k
NIAID NIH HHS R01 AI132711NIAID NIH HHS R01 AI149852NIAID NIH HHS R21 AI113470NIDDK NIH HHS K01 DK092352NIDDK NIH HHS R03 DK112004NIH HHS K01-DK092352
6 · The paper itself

Abstract

Bacterial flagella are involved in infection through their roles in host cell adhesion, cell invasion, auto-agglutination, colonization, the formation of biofilms, and the regulation and secretion of nonflagellar bacterial proteins that are involved in the virulence process. In this study, we constructed a fusion protein vaccine (FliCD) containing the Clostridioides difficile flagellar proteins FliC and FliD. The immunization of mice with FliCD induced potent IgG and IgA antibody responses against FliCD, protected mice against C. difficile infection (CDI), and decreased the C. difficile spore and toxin levels in the feces after infection. Additionally, the anti-FliCD serum inhibited the binding of C. difficile vegetative cells to HCT8 cells. These results suggest that FliCD may represent an effective vaccine candidate against CDI.

Indexed as

Clostridioides difficileClostridium InfectionsAnimalsBacterial ProteinsBacterial VaccinesClostridioidesMiceRecombinant Fusion ProteinsBacterial ProteinsBacterial VaccinesRecombinant Fusion Proteinschimeric protein vaccineClostridioides difficile infection (CDI)FliCDhyperimmune serum

Identifiers

PMID36939332
PMCPMC10112125

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.