Evidence map›Paper›PMID 36938583›Full record

ArticleChemical communications (Cambridge, England)2023

Total chemical synthesis of sumoylated histone H4 reveals negative biochemical crosstalk with histone ubiquitylation.

Sumeet K Singh, Andres Reyna, Xiaowen Xie, Haibin Mao, Meihuan Ji, Ning Zheng, Peter L Hsu, Champak Chatterjee

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical communications (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Sumeet K SinghDepartment of Chemistry, University of Washington, Seattle 98195, USA. champak1@uw.edu.ORCID http://orcid.org/0000-0003-4682-8789
Andres ReynaDepartment of Chemistry, University of Washington, Seattle 98195, USA. champak1@uw.edu.
Xiaowen XieDepartment of Pharmacology, University of Washington; Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA.ORCID http://orcid.org/0000-0002-9884-1865
Haibin MaoDepartment of Pharmacology, University of Washington; Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA.
Meihuan JiDepartment of Chemistry, University of Washington, Seattle 98195, USA. champak1@uw.edu.ORCID http://orcid.org/0000-0002-0747-7536
Ning ZhengDepartment of Pharmacology, University of Washington; Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA.
Peter L HsuDepartment of Pharmacology, University of Washington; Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA.
Champak ChatterjeeDepartment of Chemistry, University of Washington, Seattle 98195, USA. champak1@uw.edu.ORCID http://orcid.org/0000-0001-5772-4438
Howard Hughes Medical Institute · USUniversity of Washington · US

Funding

Chemical Strategies to Investigate Gene Regulation By Histone SumoylationR01GM110430 · NIGMS · UNIVERSITY OF WASHINGTON · PI CHATTERJEE, CHAMPAK · 2014 to 2022
$2.5M
Structure and Mechanism of the SET1/COMPASS H3K4 Methyltransferase ComplexR01HD097408 · NICHD · UNIVERSITY OF WASHINGTON · PI CHATTERJEE, CHAMPAK, ZHENG, NING · 2020 to 2024
$1.9M
Chemical strategies to investigate biochemical crosstalk in human chromatinR35GM149228 · NIGMS · UNIVERSITY OF WASHINGTON · PI Champak Chatterjee · 2023 to 2026
$1.6M
NICHD NIH HHS R01 HD097408NIGMS NIH HHS R01 GM110430NIGMS NIH HHS R35 GM149228
6 · The paper itself

Abstract

An efficient total chemical synthesis of site-specifically sumoylated histone H4 was undertaken to generate homogenously modified mononucleosomes. These were tested as substrates in biochemical assays with the histone H2B-specific ubiquitin ligases Rad6 and Bre1, which revealed the strong inhibition of H2B ubiquitylation by SUMO. This novel negative biochemical crosstalk between SUMO and ubiquitin was also confirmed to exist in human cells.

Indexed as

HistonesSaccharomyces cerevisiae ProteinsHumansSaccharomyces cerevisiaeUbiquitinUbiquitinationUbiquitin-Conjugating EnzymesBre1 protein, S cerevisiaeHistonesRAD6 protein, S cerevisiaeSaccharomyces cerevisiae ProteinsUbiquitinUbiquitin-Conjugating Enzymes

Identifiers

PMID36938583
PMCPMC10099519
OpenAlexW4323774915

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.