ArticlePNAS nexus2023
Crisscross multilayering of cell sheets.
Article in PNAS nexus, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Extracellular-matrix mediated stresses spatially bias myoblast fusion and myotube growth.Nature communications · 2026Article
- Fibronectin matrix remodelling modulates the active nematic dynamics of cancer-associated fibroblasts.Nature materials · 2026Article
- Liquid crystal-like self-organization of glioblastoma is associated with consistent migration for high cell densities.Scientific reports · 2026Article
- Actuation of Cell Layers in Three Dimensions.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Shaping 3D minimal model tissues with mechanical constraints to orchestrate muscle differentiation.Communications biology · 2025Article
- Myoblast Choreographic Alignment.ACS nano · 2025Article
- Intercellular friction and motility drive orientational order in cell monolayers.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Stiffness anisotropy coordinates supracellular contractility driving long-range myotube-ECM alignment.Science advances · 2024Article
- Topological defects in multi-layered swarming bacteria.Soft matter · 2024Article
- Topology-guided polar ordering of collective cell migration.Science advances · 2024Article
- Machine learning topological defects in confluent tissues.Biophysical reports · 2024Article
- Spontaneous flow created by active topological defects.The European physical journal. E, Soft matter · 2022Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrostatic skeletons such as the Hydra's consist of two stacked layers of muscle cells perpendicularly oriented. In vivo, these bilayers first assemble, and then the muscle fibers of both layers develop and organize with this crisscross orientation. In the present work, we identify an alternative mechanism of crisscross bilayering of myoblasts in vitro, which results from the prior local organization of these active cells in the initial monolayer. The myoblast sheet can be described as a contractile active nematic in which, as expected, most of the +1/2 topological defects associated with this nematic order self-propel. However, as a result of the production of extracellular matrix (ECM) by the cells, a subpopulation of these comet-like defects does not show any self-propulsion. Perpendicular bilayering occurs at these stationary defects. Cells located at the head of these defects converge toward their core where they accumulate until they start migrating on top of the tail of the first layer, while the tail cells migrate in the opposite direction under the head. Since the cells keep their initial orientations, the two stacked layers end up perpendicularly oriented. This concerted process leading to a crisscross bilayering is mediated by the secretion of ECM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.