ArticleFrontiers in cellular and infection microbiology2023
Enhancing urinary tract infection diagnosis for negative culture patients with metagenomic next-generation sequencing (mNGS).
Article in Frontiers in cellular and infection microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 2 syntheses or guidelines pooled it, 31 citations in OpenAlex.
- Molecular based diagnostic testing for urinary tract infections: the results remain unclear.World journal of urology · 2026Pooled it
- Exploring the clinical and diagnostic value of metagenomic next-generation sequencing for urinary tract infection: a systematic review and meta-analysis.BMC infectious diseases · 2024Pooled it
- Immune-related nephritis, ureteritis and cystitis secondary to immune checkpoint inhibitors: A case report and review of the literature.Experimental and therapeutic medicine · 2026Article
- Cystitis in guinea pigs (Cavia porcellus): Clinical findings and treatment outcomes.The Veterinary record · 2026Article
- Reducing culture underestimation in urine: flow cytometry-guided validation of low-dose DTT pre-treatment for biofilm dispersal.Microbiology spectrum · 2026Article
- Analytical validation of a highly accurate and reliable next-generation sequencing-based urine assay.Microbiology spectrum · 2026Article
- Metagenomic and Targeted Next-Generation Sequencing in Infectious Disease Diagnostics: Current Applications, Challenges, and Future Perspectives.Diagnostics (Basel, Switzerland) · 2026Review
- The Polymerase Chain Reaction Combined With Quantum Dot Fluorescence Analysis Method for Rapid Detection of Multiple Urinary Tract Infection Pathogens in Children.Journal of clinical laboratory analysis · 2026Article
- Ultra-deep metagenomic sequencing enables reconstruction of diverse, high quality microbial genomes from human urine samples.BMC microbiology · 2026Article
- The complexity of invasive fungal diseases in the intensive care unit: evaluation of metagenomic next-generation sequencing.Frontiers in cellular and infection microbiology · 2026Article
- Two cases of severe incision infection after cesarean section: a case report.Frontiers in medicine · 2026Article
- Metagenomic next-generation sequencing: new horizons in microbiology.Frontiers in cellular and infection microbiology · 2026Review
- Efficacy of hc-tNGS for pathogen identification for pediatric cUTIs: a real-world observational study.Frontiers in cellular and infection microbiology · 2026Observational
- The clinical application of metagenomic next-generation sequencing for invasive pulmonary aspergillosis in neutropenic patients: a multicenter retrospective study in the ICU.Frontiers in cellular and infection microbiology · 2026Article
- Case Report: Ultrasound guided puncture for type 2 diabetes mellitus combined with psoas abscess-a report of two cases.Frontiers in medicine · 2026Article
- Case Report: A rare culprit of severe pulmonary infection in children: prevotella.Frontiers in pediatrics · 2026Article
- Metagenomic sequencing enables accurate pathogen and antimicrobial susceptibility profiling in complicated UTIs in approximately four hours.Nature communications · 2025Article
- China Expert consensus on the application of metagenomic next-generation sequencing for the etiological diagnosis of infections in hematological disorders (2024).Blood science (Baltimore, Md.) · 2025Article
- Successful Treatment of Critically Severe Empyema With Bronchopleural Fistula via ECMO: A Case Report.Respirology case reports · 2025Article
- Rapid and accurate testing for urinary tract infection: new clothes for the emperor.Clinical microbiology reviews · 2025Review
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Metagenomic next-generation sequencing (mNGS) is a promising technology that allows unbiased pathogen detection and is increasingly being used for clinical diagnoses. However, its application in urinary tract infection (UTI) is still scarce. Methods: The medical records of 33 patients with suspected UTI who were admitted to the Second Hospital of Tianjin Medical University from March 2021 to July 2022 and received urine mNGS were retrospectively analyzed. The performance of mNGS and conventional urine culture in diagnosing infection and identifying causative organisms was compared, and the treatment effects were evaluated in terms of changes in urinalyses and urinary symptoms. Results: In the detection of bacteria and fungi, mNGS detected at least one pathogen in 29 (87.9%) cases, including 19 (57.6%) with positive mNGS but negative culture results and 10 (30.3%) with both mNGS and culture positive results. The remaining 4 (12.1%) patients were negative by both tests. Overall, mNGS performed better than culture (87.9% vs. 30.3%, Conclusions: mNGS is a technology that has shown clear advantages over culture, particularly in the context of mixed infections and UTIs that are difficult to diagnose and treat. It helps to improve the detection of pathogens, guide changes in treatment strategies, and is an effective complement to urine culture.
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