Evidence map›Paper›PMID 36935918›Full record

ArticlePeerJ2023

An efficient transformation method for tannin-containing sorghum.

Yuan Liang, Xuehui Yan, Jingyi Xu, Yanlong Liu, Ke Xie, Jieqin Li, Qiuwen Zhan

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Article in PeerJ, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yuan LiangCollege of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China.
Xuehui YanCollege of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China.
Jingyi XuCollege of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China.
Yanlong LiuCollege of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China.
Ke XieResearch Center of Biology and Agriculture, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.
Jieqin LiCollege of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China.
Qiuwen ZhanCollege of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Tannins are the main bottlenecks restricting the transformation efficiency of plants. Hongyingzi is a special tannin-containing sorghum cultivar used in brewing. Methods: In this study, a highly efficient microprojectile transformation system for tannin-containing sorghum was successfully exploited using immature embryos (IEs) of Hongyingzi as explants. Results: Hongyingzi presented two types of calli. Type II calli were found to be the most suitable and effective explants for transformation. After optimization of the geneticin (G418) concentration and tissue culture medium, an average transformation frequency of 27% was achieved. Molecular analyzis showed that all transgenic plants were positive and showed transgenes expression. The inheritance analyzis confirmed that the transgenes could be inherited into the next generation. Thus, we successfully established an efficient transformation system for tannin-containing sorghum and demonstrated the possibility of breaking the restriction imposed by tannins in plants.

Indexed as

SorghumTanninsEdible GrainPlants, Genetically ModifiedTransformation, GeneticTransgenesTanninsGenetic transformationParticle bombardmentTannin-containing sorghumTissue cultureTransgenic plants

Identifiers

PMID36935918
PMCPMC10022505

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